2004 12 18 0 2003 11 12 2003 11 12 Public Release 25103 emc ms(3)61CD l(3)j4E11 l(3)05592 l(3)04322 0977/09 0587/01 0203/10 0094/26 Dm0688 CT1010 CG1007 gov Emc Ach extra macrochaetae emc 24654863 0 Flybase FBgn0000575 Drosophila melanogaster taxon 7227 extra macrochaetae CG1007-PA 0 prod l(2)k08810 l(2)88/10 CT42563 CT34982 CG18608 Prod PROD proliferation disrupter prod 24655366 0 Flybase FBgn0014269 Drosophila melanogaster taxon 7227 proliferation disrupter CG18608-PA 0 emc protein interacts with prod protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-DBD-emc Gal4 DNA binding domain (DBD) fused to emc emc was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-prod Gal4 DNA activation domain (AD) fused to prod prod was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between emc and prod was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.514249029 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27604 so CG11121 CT8839 somda old med mda ami Drl So sine oculis so 17136492 0 Flybase FBgn0003460 Drosophila melanogaster taxon 7227 sine oculis CG11121-PA 0 CkIIbeta DmCkIIbeta CKII-beta1 DmCK2beta CkIIbeta1 Cask-II-b CK-IIbeta CKIIbeta betaCK2 CT41870 CT41866 CT35161 CK2beta CG15224 Andante dCKII CKIIb CK-II beta CkII CKII CK-2 CCK2 mbu kin cas CK2 And II Ds CK Casein kinase II beta subunit CkIIbeta 24641400 0 Flybase FBgn0000259 Drosophila melanogaster taxon 7227 Casein kinase II beta subunit CG15224-PD 0 so protein interacts with CkIIbeta protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-so Gal4 DNA binding domain (DBD) fused to so so was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CkIIbeta Gal4 DNA activation domain (AD) fused to CkIIbeta CkIIbeta was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between so and CkIIbeta was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.136130254 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 32499 spag l(2)k12101 CT32952 CG13570 spaghetti spag 17864228 0 Flybase FBgn0015544 Drosophila melanogaster taxon 7227 spaghetti CG13570-PA 0 cact n(2)k17003 CT18347 CG5848 dip6 cac cactus cact 24584588 0 Flybase FBgn0000250 Drosophila melanogaster taxon 7227 cactus CG5848-PA 0 spag protein interacts with cact protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-DBD-spag Gal4 DNA binding domain (DBD) fused to spag spag was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-cact Gal4 DNA activation domain (AD) fused to cact cact was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between spag and cact was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.613061969 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 25120 spir p150-Spir l(2)08327 CT30607 CT28309 CG18621 CG10076 38C.37 38C.34 Spir spire spir 28574278 0 Flybase FBgn0003475 Drosophila melanogaster taxon 7227 spire CG10076-PC 0 sax CT5838 CG1891 Brk43E Bkr43E STK-B Sax saxophone sax 24586381 0 Flybase FBgn0003317 Drosophila melanogaster taxon 7227 saxophone CG1891-PB 0 spir protein interacts with sax protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-DBD-spir Gal4 DNA binding domain (DBD) fused to spir spir was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-sax Gal4 DNA activation domain (AD) fused to sax sax was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between spir and sax was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.603840261 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 30369 Hsp83 ms(3)08445 Su(Raf)3A l(3)j5C2 E(sina)2 E(sev)3A DMHSP82 CT2354 CG1242 hsp90 hsp84 hsp83 hsp82 Hsp90 Hsp82 HSP90 HSP83 HSP82 ORF1 stc 83 Heat shock protein 83 Hsp83 17647529 0 Flybase FBgn0001233 Drosophila melanogaster taxon 7227 Heat shock protein 83 CG1242-PA 0 CG12491 CT32938 CG12491 24762383 0 Flybase FBgn0034900 Drosophila melanogaster taxon 7227 CG12491-PA 0 Hsp83 protein interacts with CG12491 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-Hsp83 Gal4 DNA activation domain (AD) fused to Hsp83 Hsp83 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG12491 Gal4 DNA binding domain (DBD) fused to CG12491 CG12491 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Hsp83 and CG12491 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.125996248 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 39928 mRpL9 CT15816 CG31478 CG4923 L9 mitochondrial ribosomal protein L9 mRpL9 28571728 0 Flybase FBgn0038319 Drosophila melanogaster taxon 7227 mitochondrial ribosomal protein L9 CG31478-PA 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 mRpL9 protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-mRpL9 Gal4 DNA activation domain (AD) fused to mRpL9 mRpL9 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between mRpL9 and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.269320677 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 30388 Hsp83 ms(3)08445 Su(Raf)3A l(3)j5C2 E(sina)2 E(sev)3A DMHSP82 CT2354 CG1242 hsp90 hsp84 hsp83 hsp82 Hsp90 Hsp82 HSP90 HSP83 HSP82 ORF1 stc 83 Heat shock protein 83 Hsp83 17647529 0 Flybase FBgn0001233 Drosophila melanogaster taxon 7227 Heat shock protein 83 CG1242-PA 0 CG8316 CT24555 CG8316 Drosophila melanogaster taxon 7227 CG8316-PA The cDNA isolated for this molecule contained only 3' untranslated sequence corresponding to GI 18859939 (Table S7, Supplementary Table). 0 Hsp83 protein interacts with CG8316 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). The cDNA isolated for CG8316 contained only 3' untranslated sequence (Table S7, Supplementary Table). 0 0 34 Gal4-AD-Hsp83 Gal4 DNA activation domain (AD) fused to Hsp83 Hsp83 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG8316 Gal4 DNA binding domain (DBD) fused to CG8316 CG8316 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. The cDNA isolated for CG8316 contained only 3' untranslated sequence (Table S7, Supplementary Table) 0 An interaction between Hsp83 and CG8316 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.013241517 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 29194 Abd-B Uab-5[Sab] l(3)89Ed DROABDB CT40560 CT36635 CT28893 CG11648 CG10291 AbdB-II iab8.9 AbdB-I tuh-3 pH189 iab-8 iab-7 iab-6 iab-5 abd-B Fab-7 Abd-b twig iab9 iab8 iab7 iab6 iab5 abdB Fab7 AbdB ABDB iab Tab Sab Mcp MCP FAB Mc Abdominal B Abd-B 24647546 0 Flybase FBgn0000015 Drosophila melanogaster taxon 7227 Abdominal B CG11648-PD 0 CG1486 CT3725 CG1486 24643689 0 Flybase FBgn0031174 Drosophila melanogaster taxon 7227 CG1486-PB 0 Abd-B protein interacts with CG1486 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-DBD-Abd-B Gal4 DNA binding domain (DBD) fused to Abd-B Abd-B was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CG1486 Gal4 DNA activation domain (AD) fused to CG1486 CG1486 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Abd-B and CG1486 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.670129372 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 36721 CG17292 CT38265 CT32726 CG17292 24582804 0 Flybase FBgn0032029 Drosophila melanogaster taxon 7227 CG17292-PB 0 ttk ttk/FTZ-F2 E(yan)100D l(3)02667 ftzf2/ttk l(3)j7B8 l(3)j2A1 CT36470 CT36468 CT36466 CG11558 1418/06 1396/14 1372/08 1325/15 1281/04 1260/10 1209/10 1209/05 1184/16 1119/04 1049/07 0702/07 0438/31 0250/25 0037/17 ttkp69 schmal ftz-f2 FTZ-F2 CT5673 CG1856 ttk69 Ttk69 TTK88 5311 5125 3540 ovs oss osn Ttk tramtrack ttk 24651713 0 Flybase FBgn0003870 Drosophila melanogaster taxon 7227 tramtrack CG1856-PC 0 CG17292 protein interacts with ttk protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-CG17292 Gal4 DNA activation domain (AD) fused to CG17292 CG17292 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-ttk Gal4 DNA binding domain (DBD) fused to ttk ttk was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG17292 and ttk was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.211021206 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 37024 cad CT5072 CG1759 38E.19 S67 Cad caudal cad 19549891 0 Flybase FBgn0000251 Drosophila melanogaster taxon 7227 caudal CG1759-PB 0 Khc l(2)k13314 l(2R)W12 l(2)W12 kinesin CT23616 CG7765 KIF5A Dmkin DmKHC kin khc Kin KHC DKH DK Kinesin heavy chain Khc 17136240 0 Flybase FBgn0001308 Drosophila melanogaster taxon 7227 Kinesin heavy chain CG7765-PA 0 cad protein interacts with Khc protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-cad Gal4 DNA binding domain (DBD) fused to cad cad was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-Khc Gal4 DNA activation domain (AD) fused to Khc Khc was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between cad and Khc was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.184453755 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 11 2003 11 12 Public Release 45057 phyl Su(Raf)2C CT28451 CG10108 SR2-3 phyllopod phyl 24653649 0 Flybase FBgn0013725 Drosophila melanogaster taxon 7227 phyllopod CG10108-PA 0 ttk ttk/FTZ-F2 E(yan)100D l(3)02667 ftzf2/ttk l(3)j7B8 l(3)j2A1 CT36470 CT36468 CT36466 CG11558 1418/06 1396/14 1372/08 1325/15 1281/04 1260/10 1209/10 1209/05 1184/16 1119/04 1049/07 0702/07 0438/31 0250/25 0037/17 ttkp69 schmal ftz-f2 FTZ-F2 CT5673 CG1856 ttk69 Ttk69 TTK88 5311 5125 3540 ovs oss osn Ttk tramtrack ttk 24651713 0 Flybase FBgn0003870 Drosophila melanogaster taxon 7227 tramtrack CG1856-PC 0 phyl protein interacts with ttk protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 1 0 34 Gal4-DBD-phyl Gal4 DNA binding domain (DBD) fused to phyl phyl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-ttk Gal4 DNA activation domain (AD) fused to ttk ttk was part of Drosophila embryo and adult cDNA libraries or was pooled from Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 An interaction between phyl and ttk was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.878800326 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 1 34 Gal4-AD-phyl Gal 4 DNA activation domain (AD) fused to phyl phyl was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-ttk Gal 4 DNA binding domain (DBD) fused to ttk ttk was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 An interaction between phyl and ttk was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.878800326 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27614 so CG11121 CT8839 somda old med mda ami Drl So sine oculis so 17136492 0 Flybase FBgn0003460 Drosophila melanogaster taxon 7227 sine oculis CG11121-PA 0 CG6688 CT20786 CG6688 24649175 0 Flybase FBgn0039038 Drosophila melanogaster taxon 7227 CG6688-PA 0 so protein interacts with CG6688 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-so Gal4 DNA binding domain (DBD) fused to so so was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CG6688 Gal4 DNA activation domain (AD) fused to CG6688 CG6688 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between so and CG6688 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.110492225 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 41219 CG3428 CT11555 CG3428 24661685 0 Flybase FBgn0036005 Drosophila melanogaster taxon 7227 CG3428-PA 0 Ras85D fs(3)05703 Su(tor)3-2 p21[Ras1] l(3)s1747 l(3)06677 E(sev)3C Dmras85D Dras85D D-ras-1 CT26623 ras85D ras85B E(faf) Draf-1 D-ras1 D-Ras1 CG9375 C-ras1 dRas1 dRAS1 Ras-1 EK3-4 Dras1 DRAS1 D-Ras ras1 RasI Ras1 RAS1 Dras DRas ras p21 Ras RTK RAS Ras oncogene at 85D Ras85D 17136430 0 Flybase FBgn0003205 Drosophila melanogaster taxon 7227 Ras oncogene at 85D CG9375-PA 0 CG3428 protein interacts with Ras85D protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-CG3428 Gal4 DNA binding domain (DBD) fused to CG3428 CG3428 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-Ras85D Gal4 DNA activation domain (AD) fused to Ras85D Ras85D was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG3428 and Ras85D was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.488319331 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 29196 Abd-B Uab-5[Sab] l(3)89Ed DROABDB CT40560 CT36635 CT28893 CG11648 CG10291 AbdB-II iab8.9 AbdB-I tuh-3 pH189 iab-8 iab-7 iab-6 iab-5 abd-B Fab-7 Abd-b twig iab9 iab8 iab7 iab6 iab5 abdB Fab7 AbdB ABDB iab Tab Sab Mcp MCP FAB Mc Abdominal B Abd-B 24647546 0 Flybase FBgn0000015 Drosophila melanogaster taxon 7227 Abdominal B CG11648-PD 0 CG31973 CT9357 CG2761 CG31973 24580513 0 Flybase FBgn0051973 Drosophila melanogaster taxon 7227 CG31973-PB 0 Abd-B protein interacts with CG31973 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-Abd-B Gal4 DNA binding domain (DBD) fused to Abd-B Abd-B was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CG31973 Gal4 DNA activation domain (AD) fused to CG31973 CG31973 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Abd-B and CG31973 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.176537519 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 43609 sop l(2)k04603 l(2)03848 Dm01335 CT18571 CG5920 RpS2 dS2 string of pearls sop 17136734 0 Flybase FBgn0004867 Drosophila melanogaster taxon 7227 string of pearls CG5920-PA 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 sop protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-sop Gal4 DNA activation domain (AD) fused to sop sop was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between sop and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.035356795 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27616 so CG11121 CT8839 somda old med mda ami Drl So sine oculis so 17136492 0 Flybase FBgn0003460 Drosophila melanogaster taxon 7227 sine oculis CG11121-PA 0 C15 Hox11-311 CT23946 CG7937 93Bal Ect5 311 C15 C15 17136732 0 Flybase FBgn0004863 Drosophila melanogaster taxon 7227 C15 CG7937-PA 0 so protein interacts with C15 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-so Gal4 DNA binding domain (DBD) fused to so so was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-C15 Gal4 DNA activation domain (AD) fused to C15 C15 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between so and C15 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.154312817 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 43036 CHIP CT16643 CG5203 CHIP 17137692 0 Flybase FBgn0027052 Drosophila melanogaster taxon 7227 CG5203-PA 0 Fmr1 EP(3)3517 CT20883 CT17036 AT24755 cg6203 CG6203 dmfr1 dfmr1 dFXR1 dFMRP dFMR1 dfxr dFXR FMR1 FXR Fragile-X-related Fmr1 24645602 0 Flybase FBgn0028734 Drosophila melanogaster taxon 7227 CG6203-PB 0 CHIP protein interacts with Fmr1 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-AD-CHIP Gal4 DNA activation domain (AD) fused to CHIP CHIP was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-Fmr1 Gal4 DNA binding domain (DBD) fused to Fmr1 Fmr1 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CHIP and Fmr1 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.513748032 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 26985 ng1 EG:96G10.5 CG10781 nested CT9307 ng-1 ngp SC1 ng new glue 1 ng1 17136358 0 Flybase FBgn0002933 Drosophila melanogaster taxon 7227 new glue 1 CG10781-PA 0 ea CT15808 c-SP24 CG4920 SP24 easter ea 24647107 0 Flybase FBgn0000533 Drosophila melanogaster taxon 7227 easter CG4920-PA 0 ng1 protein interacts with ea protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-DBD-ng1 Gal4 DNA binding domain (DBD) fused to ng1 ng1 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-ea Gal4 DNA activation domain (AD) fused to ea ea was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between ng1 and ea was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.68259679 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27153 osk CT41904 CT30527 CG10901 oskar osk 24645205 0 Flybase FBgn0003015 Drosophila melanogaster taxon 7227 oskar CG10901-PA 0 Myo10A AAF47980 CT7104 CG2174 CG2136 DmXV 10A unconventional myosin class XV Myo10A 24641148 0 Flybase FBgn0030252 Drosophila melanogaster taxon 7227 unconventional myosin class XV CG2174-PA 0 osk protein interacts with Myo10A protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-osk Gal4 DNA activation domain (AD) fused to osk osk was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-Myo10A Gal4 DNA binding domain (DBD) fused to Myo10A Myo10A was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between osk and Myo10A was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.130834877 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 29506 CG11798 CT23317 CG11798 20130007 0 Flybase FBgn0033992 Drosophila melanogaster taxon 7227 CG11798-PA 0 Hsp83 ms(3)08445 Su(Raf)3A l(3)j5C2 E(sina)2 E(sev)3A DMHSP82 CT2354 CG1242 hsp90 hsp84 hsp83 hsp82 Hsp90 Hsp82 HSP90 HSP83 HSP82 ORF1 stc 83 Heat shock protein 83 Hsp83 17647529 0 Flybase FBgn0001233 Drosophila melanogaster taxon 7227 Heat shock protein 83 CG1242-PA 0 CG11798 protein interacts with Hsp83 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-CG11798 Gal4 DNA binding domain (DBD) fused to CG11798 CG11798 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-Hsp83 Gal4 DNA activation domain (AD) fused to Hsp83 Hsp83 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG11798 and Hsp83 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.089781959 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 37880 ttk ttk/FTZ-F2 E(yan)100D l(3)02667 ftzf2/ttk l(3)j7B8 l(3)j2A1 CT36470 CT36468 CT36466 CG11558 1418/06 1396/14 1372/08 1325/15 1281/04 1260/10 1209/10 1209/05 1184/16 1119/04 1049/07 0702/07 0438/31 0250/25 0037/17 ttkp69 schmal ftz-f2 FTZ-F2 CT5673 CG1856 ttk69 Ttk69 TTK88 5311 5125 3540 ovs oss osn Ttk tramtrack ttk 24651713 0 Flybase FBgn0003870 Drosophila melanogaster taxon 7227 tramtrack CG1856-PC 0 CG3371 CT11327 EP3592 CG3371 24654832 0 Flybase FBgn0035153 Drosophila melanogaster taxon 7227 CG3371-PA 0 ttk protein interacts with CG3371 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-ttk Gal4 DNA binding domain (DBD) fused to ttk ttk was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CG3371 Gal4 DNA activation domain (AD) fused to CG3371 CG3371 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between ttk and CG3371 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.211021206 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 29195 Abd-B Uab-5[Sab] l(3)89Ed DROABDB CT40560 CT36635 CT28893 CG11648 CG10291 AbdB-II iab8.9 AbdB-I tuh-3 pH189 iab-8 iab-7 iab-6 iab-5 abd-B Fab-7 Abd-b twig iab9 iab8 iab7 iab6 iab5 abdB Fab7 AbdB ABDB iab Tab Sab Mcp MCP FAB Mc Abdominal B Abd-B 24647546 0 Flybase FBgn0000015 Drosophila melanogaster taxon 7227 Abdominal B CG11648-PD 0 Ef2b CT43395 CT7410 CG2238 EF-2b eEF2 EF-2 Ef2 EF2 Elongation factor 2b Ef2b 24585713 0 Flybase FBgn0000559 Drosophila melanogaster taxon 7227 Elongation factor 2b CG2238-PC 0 Abd-B protein interacts with Ef2b protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-Abd-B Gal4 DNA binding domain (DBD) fused to Abd-B Abd-B was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-Ef2b Gal4 DNA activation domain (AD) fused to Ef2b Ef2b was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Abd-B and Ef2b was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.055741887 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27607 so CG11121 CT8839 somda old med mda ami Drl So sine oculis so 17136492 0 Flybase FBgn0003460 Drosophila melanogaster taxon 7227 sine oculis CG11121-PA 0 VhaAC39 vhaAC39 CT9953 CG2934 VhaAC39 18543319 0 Flybase FBgn0028665 Drosophila melanogaster taxon 7227 CG2934-PA 0 so protein interacts with VhaAC39 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-DBD-so Gal4 DNA binding domain (DBD) fused to so so was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-VhaAC39 Gal4 DNA activation domain (AD) fused to VhaAC39 VhaAC39 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between so and VhaAC39 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.562803178 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 43034 CHIP CT16643 CG5203 CHIP 17137692 0 Flybase FBgn0027052 Drosophila melanogaster taxon 7227 CG5203-PA 0 cact n(2)k17003 CT18347 CG5848 dip6 cac cactus cact 24584588 0 Flybase FBgn0000250 Drosophila melanogaster taxon 7227 cactus CG5848-PA 0 CHIP protein interacts with cact protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-CHIP Gal4 DNA binding domain (DBD) fused to CHIP CHIP was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-cact Gal4 DNA activation domain (AD) fused to cact cact was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CHIP and cact was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.211232818 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27602 so CG11121 CT8839 somda old med mda ami Drl So sine oculis so 17136492 0 Flybase FBgn0003460 Drosophila melanogaster taxon 7227 sine oculis CG11121-PA 0 CG1135 CT1834 CG1135 21356337 0 Flybase FBgn0035489 Drosophila melanogaster taxon 7227 CG1135-PA 0 so protein interacts with CG1135 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-so Gal4 DNA binding domain (DBD) fused to so so was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CG1135 Gal4 DNA activation domain (AD) fused to CG1135 CG1135 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between so and CG1135 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.047175407 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 39514 EG:84H4.4 EG:84H4.4 CT10316 CG3081 23397623 0 Flybase FBgn0025613 Drosophila melanogaster taxon 7227 CG3081-PA 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 EG:84H4.4 protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-EG:84H4.4 Gal4 DNA activation domain (AD) fused to EG:84H4.4 EG:84H4.4 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between EG:84H4.4 and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.150035229 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27605 so CG11121 CT8839 somda old med mda ami Drl So sine oculis so 17136492 0 Flybase FBgn0003460 Drosophila melanogaster taxon 7227 sine oculis CG11121-PA 0 CG15459 CT35528 CG15459 24643497 0 Flybase FBgn0031108 Drosophila melanogaster taxon 7227 CG15459-PA 0 so protein interacts with CG15459 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-so Gal4 DNA binding domain (DBD) fused to so so was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CG15459 Gal4 DNA activation domain (AD) fused to CG15459 CG15459 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between so and CG15459 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.065199161 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 25694 D19B CT28859 CG10270 D19B 17137444 0 Flybase FBgn0022699 Drosophila melanogaster taxon 7227 CG10270-PA 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 D19B protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-D19B Gal4 DNA activation domain (AD) fused to D19B D19B was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between D19B and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.286045554 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 35100 BG:DS01759.2 BG:DS01759.2 CT35241 CG15293 19921326 0 Flybase FBgn0028526 Drosophila melanogaster taxon 7227 CG15293-PA 0 shg shg/DE-Cad l(2)k10220 l(2)k03401 E-cadherin E-Cadherin l(2)10469 cadherin DE[cyto] Cadherin CT12481 DE-cad DE-Cad DE-CAD CG3722 gp150 E-cad DECad DCAD2 D-cad Ecad DCad CadE CADH Shg DEC Cad DE shotgun shg 17136470 0 Flybase FBgn0003391 Drosophila melanogaster taxon 7227 shotgun CG3722-PA 0 BG:DS01759.2 protein interacts with shg protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-DBD-BG:DS01759.2 Gal4 DNA binding domain (DBD) fused to BG:DS01759.2 BG:DS01759.2 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-shg Gal4 DNA activation domain (AD) fused to shg shg was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between BG:DS01759.2 and shg was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.506790752 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 36460 pnt pointed-P2 pointed-P1 pointedP2 l(3)07825 DMPOINT1A l(3)j1B7 E(E2F)3D Ets58AB D-ets-2 D-Ets-2 CT37466 CG17077 0998/12 0608/07 0123/09 ets94F Ets94F POINT EY3-1 EK3-2 Ets2 ETS2 3520 ptd pointed pnt 24649295 0 Flybase FBgn0003118 Drosophila melanogaster taxon 7227 pointed CG17077-PC 0 ATPsyn-b CT24393 CG8189 ATP synthase, subunit b ATPsyn-b 24661888 0 Flybase FBgn0019644 Drosophila melanogaster taxon 7227 ATP synthase, subunit b CG8189-PB 0 pnt protein interacts with ATPsyn-b protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-pnt Gal4 DNA binding domain (DBD) fused to pnt pnt was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-ATPsyn-b Gal4 DNA activation domain (AD) fused to ATPsyn-b ATPsyn-b was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between pnt and ATPsyn-b was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.365368071 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 29200 Abd-B Uab-5[Sab] l(3)89Ed DROABDB CT40560 CT36635 CT28893 CG11648 CG10291 AbdB-II iab8.9 AbdB-I tuh-3 pH189 iab-8 iab-7 iab-6 iab-5 abd-B Fab-7 Abd-b twig iab9 iab8 iab7 iab6 iab5 abdB Fab7 AbdB ABDB iab Tab Sab Mcp MCP FAB Mc Abdominal B Abd-B 24647546 0 Flybase FBgn0000015 Drosophila melanogaster taxon 7227 Abdominal B CG11648-PD 0 Pk17E CT21577 CG7001 bin4 Pk4 Bin4 BIN4 3-10 Protein kinase-like 17E Pk17E 24643118 0 Flybase FBgn0004462 Drosophila melanogaster taxon 7227 Protein kinase-like 17E CG7001-PA 0 Abd-B protein interacts with Pk17E protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-Abd-B Gal4 DNA binding domain (DBD) fused to Abd-B Abd-B was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-Pk17E Gal4 DNA activation domain (AD) fused to Pk17E Pk17E was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Abd-B and Pk17E was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.040518208 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27612 so CG11121 CT8839 somda old med mda ami Drl So sine oculis so 17136492 0 Flybase FBgn0003460 Drosophila melanogaster taxon 7227 sine oculis CG11121-PA 0 EG:52C10.1 EG:52C10.1 CT16163 CG5033 24654626 0 Flybase FBgn0028744 Drosophila melanogaster taxon 7227 CG5033-PB 0 so protein interacts with EG:52C10.1 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-DBD-so Gal4 DNA binding domain (DBD) fused to so so was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-EG:52C10.1 Gal4 DNA activation domain (AD) fused to EG:52C10.1 EG:52C10.1 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between so and EG:52C10.1 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.559069484 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 26567 tup l(2)37Aa l(2)E41 Islet 1 CT29726 CG10619 islet isl Isl tailup tup 17136568 0 Flybase FBgn0003896 Drosophila melanogaster taxon 7227 tailup CG10619-PA 0 Fmr1 EP(3)3517 CT20883 CT17036 AT24755 cg6203 CG6203 dmfr1 dfmr1 dFXR1 dFMRP dFMR1 dfxr dFXR FMR1 FXR Fragile-X-related Fmr1 24645602 0 Flybase FBgn0028734 Drosophila melanogaster taxon 7227 CG6203-PB 0 tup protein interacts with Fmr1 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-AD-tup Gal4 DNA activation domain (AD) fused to tup tup was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-Fmr1 Gal4 DNA binding domain (DBD) fused to Fmr1 Fmr1 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between tup and Fmr1 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.91159076 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 29199 Abd-B Uab-5[Sab] l(3)89Ed DROABDB CT40560 CT36635 CT28893 CG11648 CG10291 AbdB-II iab8.9 AbdB-I tuh-3 pH189 iab-8 iab-7 iab-6 iab-5 abd-B Fab-7 Abd-b twig iab9 iab8 iab7 iab6 iab5 abdB Fab7 AbdB ABDB iab Tab Sab Mcp MCP FAB Mc Abdominal B Abd-B 24647546 0 Flybase FBgn0000015 Drosophila melanogaster taxon 7227 Abdominal B CG11648-PD 0 CSN6 CT21470 CG6932 Dch6 CH6 COP9 complex homolog subunit 6 CSN6 17738125 0 Flybase FBgn0028837 Drosophila melanogaster taxon 7227 COP9 complex homolog subunit 6 CG6932-PA 0 Abd-B protein interacts with CSN6 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-Abd-B Gal4 DNA binding domain (DBD) fused to Abd-B Abd-B was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CSN6 Gal4 DNA activation domain (AD) fused to CSN6 CSN6 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Abd-B and CSN6 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.237968733 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 41750 CG3977 CT13228 CG3977 24640170 0 Flybase FBgn0029886 Drosophila melanogaster taxon 7227 CG3977-PA 0 ash1 l(3)SG29a CT25366 CG8887 ash-1 dash Ash1 ASH1 ash absent, small, or homeotic discs 1 ash1 17737643 0 Flybase FBgn0005386 Drosophila melanogaster taxon 7227 absent, small, or homeotic discs 1 CG8887-PA 0 CG3977 protein interacts with ash1 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-DBD-CG3977 Gal4 DNA binding domain (DBD) fused to CG3977 CG3977 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-ash1 Gal4 DNA activation domain (AD) fused to ash1 ash1 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG3977 and ash1 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.529221485 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 29042 arm beta-catenin l(1)G0234 l(1)G0192 b-catenin EG:86E4.6 l(1)arm l(1)2Bv catenin CT36522 CT36518 CT12773 CG11579 Arm ARM armadillo arm 24639206 0 Flybase FBgn0000117 Drosophila melanogaster taxon 7227 armadillo CG11579-PC 0 CG9541 CT26990 CG9541 24582962 0 Flybase FBgn0032083 Drosophila melanogaster taxon 7227 CG9541-PA 0 arm protein interacts with CG9541 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-DBD-arm Gal4 DNA binding domain (DBD) fused to arm arm was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CG9541 Gal4 DNA activation domain (AD) fused to CG9541 CG9541 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between arm and CG9541 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.711552087 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27613 so CG11121 CT8839 somda old med mda ami Drl So sine oculis so 17136492 0 Flybase FBgn0003460 Drosophila melanogaster taxon 7227 sine oculis CG11121-PA 0 CG5466 CT16675 CG5466 24648461 0 Flybase FBgn0038815 Drosophila melanogaster taxon 7227 CG5466-PA 0 so protein interacts with CG5466 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-so Gal4 DNA binding domain (DBD) fused to so so was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CG5466 Gal4 DNA activation domain (AD) fused to CG5466 CG5466 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between so and CG5466 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.126354285 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 43209 CG5397 CT16527 CG5397 19920512 0 Flybase FBgn0031327 Drosophila melanogaster taxon 7227 CG5397-PA 0 cact n(2)k17003 CT18347 CG5848 dip6 cac cactus cact 24584588 0 Flybase FBgn0000250 Drosophila melanogaster taxon 7227 cactus CG5848-PA 0 CG5397 protein interacts with cact protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-CG5397 Gal4 DNA binding domain (DBD) fused to CG5397 CG5397 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-cact Gal4 DNA activation domain (AD) fused to cact cact was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG5397 and cact was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.444722359 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27076 dynactin-subunit-p25 DS00929.1 CT30375 CG10846 dynactin-subunit-p25 24584372 0 Flybase FBgn0040228 Drosophila melanogaster taxon 7227 CG10846-PA 0 ovo shavenbaby fs(1)K1237 Shavenbaby Fs(1)K1237 Fs(1)K1103 Fs(1)K155 fs(1)M38 ovo/svb fs(1)M1 CT36311 CT21113 CG6824 svb Svb Ovo OVO ovo ovo 24639804 0 Flybase FBgn0003028 Drosophila melanogaster taxon 7227 ovo CG6824-PA 0 dynactin-subunit-p25 protein interacts with ovo protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-dynactin-subunit-p25 Gal4 DNA binding domain (DBD) fused to dynactin-subunit-p25 dynactin-subunit-p25 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-ovo Gal4 DNA activation domain (AD) fused to ovo ovo was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between dynactin-subunit-p25 and ovo was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.408330417 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 36595 CG17168 CT35322 CG17168 21357185 0 Flybase FBgn0039943 Drosophila melanogaster taxon 7227 CG17168 gene product 0 ttk ttk/FTZ-F2 E(yan)100D l(3)02667 ftzf2/ttk l(3)j7B8 l(3)j2A1 CT36470 CT36468 CT36466 CG11558 1418/06 1396/14 1372/08 1325/15 1281/04 1260/10 1209/10 1209/05 1184/16 1119/04 1049/07 0702/07 0438/31 0250/25 0037/17 ttkp69 schmal ftz-f2 FTZ-F2 CT5673 CG1856 ttk69 Ttk69 TTK88 5311 5125 3540 ovs oss osn Ttk tramtrack ttk 24651713 0 Flybase FBgn0003870 Drosophila melanogaster taxon 7227 tramtrack CG1856-PC 0 CG17168 protein interacts with ttk protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-CG17168 Gal4 DNA binding domain (DBD) fused to CG17168 CG17168 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-ttk Gal4 DNA activation domain (AD) fused to ttk ttk was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG17168 and ttk was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.429158908 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 37383 14-3-3zeta l(2)07103 D14-3-3 CT39748 CT39744 CG17870 14-3-3 leo 549 2G1 14-3-3zeta 28573324 0 Flybase FBgn0004907 Drosophila melanogaster taxon 7227 CG17870-PG 0 csw l(1)GA114 l(1)G0170 E(sev)1A Csw/Shp2 l(1)csw l(1)2Dd l(1)2Db CT43319 CT37554 CT13063 CG3954 19-106 Csw CSW corkscrew csw 24639268 0 Flybase FBgn0000382 Drosophila melanogaster taxon 7227 corkscrew CG3954-PC 0 14-3-3zeta protein interacts with csw protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-14-3-3zeta Gal4 DNA activation domain (AD) fused to 14-3-3zeta 14-3-3zeta was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-csw Gal4 DNA binding domain (DBD) fused to csw csw was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between 14-3-3zeta and csw was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.216797762 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 45049 dpp l(2)k17036 l(2)10638 l(2)22Fa TGF-beta M(2)23AB M(2)LS1 CT9505 CG9885 TGF-b Hin-d DPP-C shv blk Dpp DPP ho Tg decapentaplegic dpp 24581146 0 Flybase FBgn0000490 Drosophila melanogaster taxon 7227 decapentaplegic CG9885-PE 0 E5 CT28119 CT27952 CG9930 E5 E5 17864460 0 Flybase FBgn0008646 Drosophila melanogaster taxon 7227 E5 CG9930-PA 0 dpp protein interacts with E5 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-dpp Gal4 DNA activation domain (AD) fused to dpp dpp was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-E5 Gal4 DNA binding domain (DBD) fused to E5 E5 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between dpp and E5 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.399461661 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27606 so CG11121 CT8839 somda old med mda ami Drl So sine oculis so 17136492 0 Flybase FBgn0003460 Drosophila melanogaster taxon 7227 sine oculis CG11121-PA 0 CG17265 CT35453 CG17265 24581314 0 Flybase FBgn0031488 Drosophila melanogaster taxon 7227 CG17265-PA 0 so protein interacts with CG17265 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-so Gal4 DNA binding domain (DBD) fused to so so was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CG17265 Gal4 DNA activation domain (AD) fused to CG17265 CG17265 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between so and CG17265 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.065199161 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 29197 Abd-B Uab-5[Sab] l(3)89Ed DROABDB CT40560 CT36635 CT28893 CG11648 CG10291 AbdB-II iab8.9 AbdB-I tuh-3 pH189 iab-8 iab-7 iab-6 iab-5 abd-B Fab-7 Abd-b twig iab9 iab8 iab7 iab6 iab5 abdB Fab7 AbdB ABDB iab Tab Sab Mcp MCP FAB Mc Abdominal B Abd-B 24647546 0 Flybase FBgn0000015 Drosophila melanogaster taxon 7227 Abdominal B CG11648-PD 0 CG33207 CT34693 CG14874 pxb 28571743 0 Flybase FBgn0053207 Drosophila melanogaster taxon 7227 CG33207-PB 0 Abd-B protein interacts with CG33207 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-Abd-B Gal4 DNA binding domain (DBD) fused to Abd-B Abd-B was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CG33207 Gal4 DNA activation domain (AD) fused to CG33207 CG33207 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Abd-B and CG33207 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.345621703 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 25607 CG10263 CT28843 CG10263 28574352 0 Flybase FBgn0032812 Drosophila melanogaster taxon 7227 CG10263-PD 0 ttk ttk/FTZ-F2 E(yan)100D l(3)02667 ftzf2/ttk l(3)j7B8 l(3)j2A1 CT36470 CT36468 CT36466 CG11558 1418/06 1396/14 1372/08 1325/15 1281/04 1260/10 1209/10 1209/05 1184/16 1119/04 1049/07 0702/07 0438/31 0250/25 0037/17 ttkp69 schmal ftz-f2 FTZ-F2 CT5673 CG1856 ttk69 Ttk69 TTK88 5311 5125 3540 ovs oss osn Ttk tramtrack ttk 24651713 0 Flybase FBgn0003870 Drosophila melanogaster taxon 7227 tramtrack CG1856-PC 0 CG10263 protein interacts with ttk protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-CG10263 Gal4 DNA activation domain (AD) fused to CG10263 CG10263 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-ttk Gal4 DNA binding domain (DBD) fused to ttk ttk was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG10263 and ttk was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.097203599 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 38879 CG2747 CT42873 CT9341 CG2747 24644960 0 Flybase FBgn0037541 Drosophila melanogaster taxon 7227 CG2747-PB 0 csw l(1)GA114 l(1)G0170 E(sev)1A Csw/Shp2 l(1)csw l(1)2Dd l(1)2Db CT43319 CT37554 CT13063 CG3954 19-106 Csw CSW corkscrew csw 24639268 0 Flybase FBgn0000382 Drosophila melanogaster taxon 7227 corkscrew CG3954-PC 0 CG2747 protein interacts with csw protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-AD-CG2747 Gal4 DNA activation domain (AD) fused to CG2747 CG2747 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-csw Gal4 DNA binding domain (DBD) fused to csw csw was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG2747 and csw was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.671806636 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 37889 ttk ttk/FTZ-F2 E(yan)100D l(3)02667 ftzf2/ttk l(3)j7B8 l(3)j2A1 CT36470 CT36468 CT36466 CG11558 1418/06 1396/14 1372/08 1325/15 1281/04 1260/10 1209/10 1209/05 1184/16 1119/04 1049/07 0702/07 0438/31 0250/25 0037/17 ttkp69 schmal ftz-f2 FTZ-F2 CT5673 CG1856 ttk69 Ttk69 TTK88 5311 5125 3540 ovs oss osn Ttk tramtrack ttk 24651713 0 Flybase FBgn0003870 Drosophila melanogaster taxon 7227 tramtrack CG1856-PC 0 CG7200 CT22215 CG7200 19921472 0 Flybase FBgn0032671 Drosophila melanogaster taxon 7227 CG7200-PA 0 ttk protein interacts with CG7200 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-ttk Gal4 DNA binding domain (DBD) fused to ttk ttk was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CG7200 Gal4 DNA activation domain (AD) fused to CG7200 CG7200 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between ttk and CG7200 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.184228652 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27609 so CG11121 CT8839 somda old med mda ami Drl So sine oculis so 17136492 0 Flybase FBgn0003460 Drosophila melanogaster taxon 7227 sine oculis CG11121-PA 0 BG:DS01068.2 CT22973 CG7469 CG33090 28574174 0 Flybase FBgn0028916 Drosophila melanogaster taxon 7227 CG33090-PA 0 so protein interacts with BG:DS01068.2 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-so Gal4 DNA binding domain (DBD) fused to so so was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-BG:DS01068.2 Gal4 DNA activation domain (AD) fused to BG:DS01068.2 BG:DS01068.2 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between so and BG:DS01068.2 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.340269537 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 26854 Lim3 l(2)37Bd CT29991 CG10699 l(2)Bd lim3 Bd Lim3 24585132 0 Flybase FBgn0002023 Drosophila melanogaster taxon 7227 CG10699-PB 0 Fmr1 EP(3)3517 CT20883 CT17036 AT24755 cg6203 CG6203 dmfr1 dfmr1 dFXR1 dFMRP dFMR1 dfxr dFXR FMR1 FXR Fragile-X-related Fmr1 24645602 0 Flybase FBgn0028734 Drosophila melanogaster taxon 7227 CG6203-PB 0 Lim3 protein interacts with Fmr1 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-AD-Lim3 Gal4 DNA activation domain (AD) fused to Lim3 Lim3 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-Fmr1 Gal4 DNA binding domain (DBD) fused to Fmr1 Fmr1 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Lim3 and Fmr1 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.595192515 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27608 so CG11121 CT8839 somda old med mda ami Drl So sine oculis so 17136492 0 Flybase FBgn0003460 Drosophila melanogaster taxon 7227 sine oculis CG11121-PA 0 CG32940 CG32940 28573175 0 Flybase FBgn0052940 Drosophila melanogaster taxon 7227 CG32940-PB 0 so protein interacts with CG32940 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-so Gal4 DNA binding domain (DBD) fused to so so was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CG32940 Gal4 DNA activation domain (AD) fused to CG32940 CG32940 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between so and CG32940 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.068708324 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 38557 CG2199 CT7250 CG2199 24655153 0 Flybase FBgn0035213 Drosophila melanogaster taxon 7227 CG2199-PB 0 Fmr1 EP(3)3517 CT20883 CT17036 AT24755 cg6203 CG6203 dmfr1 dfmr1 dFXR1 dFMRP dFMR1 dfxr dFXR FMR1 FXR Fragile-X-related Fmr1 24645602 0 Flybase FBgn0028734 Drosophila melanogaster taxon 7227 CG6203-PB 0 CG2199 protein interacts with Fmr1 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-CG2199 Gal4 DNA activation domain (AD) fused to CG2199 CG2199 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-Fmr1 Gal4 DNA binding domain (DBD) fused to Fmr1 Fmr1 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG2199 and Fmr1 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.069599182 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 31039 CG12768 CT31125 CG12768 24668820 0 Flybase FBgn0037206 Drosophila melanogaster taxon 7227 CG12768-PA 0 Fmr1 EP(3)3517 CT20883 CT17036 AT24755 cg6203 CG6203 dmfr1 dfmr1 dFXR1 dFMRP dFMR1 dfxr dFXR FMR1 FXR Fragile-X-related Fmr1 24645602 0 Flybase FBgn0028734 Drosophila melanogaster taxon 7227 CG6203-PB 0 CG12768 protein interacts with Fmr1 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-CG12768 Gal4 DNA activation domain (AD) fused to CG12768 CG12768 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-Fmr1 Gal4 DNA binding domain (DBD) fused to Fmr1 Fmr1 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG12768 and Fmr1 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.355769228 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27617 so CG11121 CT8839 somda old med mda ami Drl So sine oculis so 17136492 0 Flybase FBgn0003460 Drosophila melanogaster taxon 7227 sine oculis CG11121-PA 0 gro E(spl)gro E(spl)[2] E(spl)-WD l(3)gro Groucho CT41917 CT24657 m9/m10 m9-m10 m9,m10 En-spl CG8384 m9/10 dGro m10 Gro m9 groucho gro 24650241 0 Flybase FBgn0001139 Drosophila melanogaster taxon 7227 groucho CG8384-PD 0 so protein interacts with gro protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-DBD-so Gal4 DNA binding domain (DBD) fused to so so was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-gro Gal4 DNA activation domain (AD) fused to gro gro was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between so and gro was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.871741303 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27151 osk CT41904 CT30527 CG10901 oskar osk 24645205 0 Flybase FBgn0003015 Drosophila melanogaster taxon 7227 oskar CG10901-PA 0 CG15185 CT35100 CG15185 24644626 0 Flybase FBgn0037449 Drosophila melanogaster taxon 7227 CG15185-PA 0 osk protein interacts with CG15185 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-DBD-osk Gal4 DNA binding domain (DBD) fused to osk osk was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CG15185 Gal4 DNA activation domain (AD) fused to CG15185 CG15185 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between osk and CG15185 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.768632353 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 34396 EG:63B12.5 EG:63B12.5 CT34628 CG14815 24639189 0 Flybase FBgn0023516 Drosophila melanogaster taxon 7227 CG14815-PB 0 ttk ttk/FTZ-F2 E(yan)100D l(3)02667 ftzf2/ttk l(3)j7B8 l(3)j2A1 CT36470 CT36468 CT36466 CG11558 1418/06 1396/14 1372/08 1325/15 1281/04 1260/10 1209/10 1209/05 1184/16 1119/04 1049/07 0702/07 0438/31 0250/25 0037/17 ttkp69 schmal ftz-f2 FTZ-F2 CT5673 CG1856 ttk69 Ttk69 TTK88 5311 5125 3540 ovs oss osn Ttk tramtrack ttk 24651713 0 Flybase FBgn0003870 Drosophila melanogaster taxon 7227 tramtrack CG1856-PC 0 EG:63B12.5 protein interacts with ttk protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-EG:63B12.5 Gal4 DNA activation domain (AD) fused to EG:63B12.5 EG:63B12.5 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-ttk Gal4 DNA binding domain (DBD) fused to ttk ttk was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between EG:63B12.5 and ttk was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.483700091 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27526 CG11086 CT8879 CG11086 20129739 0 Flybase FBgn0033153 Drosophila melanogaster taxon 7227 CG11086-PA 0 so CG11121 CT8839 somda old med mda ami Drl So sine oculis so 17136492 0 Flybase FBgn0003460 Drosophila melanogaster taxon 7227 sine oculis CG11121-PA 0 CG11086 protein interacts with so protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-CG11086 Gal4 DNA activation domain (AD) fused to CG11086 CG11086 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-so Gal4 DNA binding domain (DBD) fused to so so was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG11086 and so was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.331000587 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 40253 CG3203 CT10544 CT10530 CG3203 24640206 0 Flybase FBgn0029897 Drosophila melanogaster taxon 7227 CG3203-PC 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 CG3203 protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-CG3203 Gal4 DNA activation domain (AD) fused to CG3203 CG3203 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG3203 and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.041246635 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 40622 CG32527 anon-19Fb tu2 CG32527 24643713 0 Flybase FBgn0052527 Drosophila melanogaster taxon 7227 CG32527-PC 0 cact n(2)k17003 CT18347 CG5848 dip6 cac cactus cact 24584588 0 Flybase FBgn0000250 Drosophila melanogaster taxon 7227 cactus CG5848-PA 0 CG32527 protein interacts with cact protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-CG32527 Gal4 DNA binding domain (DBD) fused to CG32527 CG32527 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-cact Gal4 DNA activation domain (AD) fused to cact cact was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG32527 and cact was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.322129183 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27152 osk CT41904 CT30527 CG10901 oskar osk 24645205 0 Flybase FBgn0003015 Drosophila melanogaster taxon 7227 oskar CG10901-PA 0 CG18398 CT29290 CG18398 24585079 0 Flybase FBgn0032728 Drosophila melanogaster taxon 7227 CG18398-PA 0 osk protein interacts with CG18398 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-osk Gal4 DNA activation domain (AD) fused to osk osk was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG18398 Gal4 DNA binding domain (DBD) fused to CG18398 CG18398 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between osk and CG18398 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.177610735 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 29198 Abd-B Uab-5[Sab] l(3)89Ed DROABDB CT40560 CT36635 CT28893 CG11648 CG10291 AbdB-II iab8.9 AbdB-I tuh-3 pH189 iab-8 iab-7 iab-6 iab-5 abd-B Fab-7 Abd-b twig iab9 iab8 iab7 iab6 iab5 abdB Fab7 AbdB ABDB iab Tab Sab Mcp MCP FAB Mc Abdominal B Abd-B 24647546 0 Flybase FBgn0000015 Drosophila melanogaster taxon 7227 Abdominal B CG11648-PD 0 CG5469 CT42571 CT17320 CG5469 24655158 0 Flybase FBgn0034372 Drosophila melanogaster taxon 7227 CG5469-PC 0 Abd-B protein interacts with CG5469 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-Abd-B Gal4 DNA binding domain (DBD) fused to Abd-B Abd-B was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CG5469 Gal4 DNA activation domain (AD) fused to CG5469 CG5469 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Abd-B and CG5469 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.370517798 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 32345 CG13474 CT32839 CG13474 24664257 0 Flybase FBgn0036439 Drosophila melanogaster taxon 7227 CG13474-PA 0 BicD CT20532 CG6605 Bic[D] Bic-D BIC-D Bicaudal D BicD 24584833 0 Flybase FBgn0000183 Drosophila melanogaster taxon 7227 Bicaudal D CG6605-PA 0 CG13474 protein interacts with BicD protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-CG13474 Gal4 DNA binding domain (DBD) fused to CG13474 CG13474 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-BicD Gal4 DNA activation domain (AD) fused to BicD BicD was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG13474 and BicD was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.217850823 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 33905 CG14542 CT34272 CG14542 24650245 0 Flybase FBgn0039402 Drosophila melanogaster taxon 7227 CG14542-PA 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 CG14542 protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-CG14542 Gal4 DNA activation domain (AD) fused to CG14542 CG14542 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG14542 and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.178935037 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 32913 tor CT3198 CG1389 splc Tor torso tor 17136542 0 Flybase FBgn0003733 Drosophila melanogaster taxon 7227 torso CG1389-PA 0 CG4289 CT14054 CG4289 21355205 0 Flybase FBgn0037020 Drosophila melanogaster taxon 7227 CG4289-PA 0 tor protein interacts with CG4289 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-AD-tor Gal4 DNA activation domain (AD) fused to tor tor was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG4289 Gal4 DNA binding domain (DBD) fused to CG4289 CG4289 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between tor and CG4289 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.551727618 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 43654 drk l(2)k13809 l(2)10626 Su(sev)R1 Grb2/drk E(sev)2B CT18920 CG6033 P1112 crkl 24/1 Drk DRK downstream of receptor kinase drk 24653406 0 Flybase FBgn0004638 Drosophila melanogaster taxon 7227 downstream of receptor kinase CG6033-PF 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 drk protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-drk Gal4 DNA activation domain (AD) fused to drk drk was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between drk and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.160466738 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27615 so CG11121 CT8839 somda old med mda ami Drl So sine oculis so 17136492 0 Flybase FBgn0003460 Drosophila melanogaster taxon 7227 sine oculis CG11121-PA 0 CG7878 CT23818 cg7878 CG7878 21355075 0 Flybase FBgn0037549 Drosophila melanogaster taxon 7227 CG7878-PA 0 so protein interacts with CG7878 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-so Gal4 DNA binding domain (DBD) fused to so so was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CG7878 Gal4 DNA activation domain (AD) fused to CG7878 CG7878 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between so and CG7878 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.233688303 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27610 so CG11121 CT8839 somda old med mda ami Drl So sine oculis so 17136492 0 Flybase FBgn0003460 Drosophila melanogaster taxon 7227 sine oculis CG11121-PA 0 RhoGAP93B CT11517 CG3421 RhoGAP93B 24648617 0 Flybase FBgn0038853 Drosophila melanogaster taxon 7227 CG3421-PA 0 so protein interacts with RhoGAP93B protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-so Gal4 DNA binding domain (DBD) fused to so so was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-RhoGAP93B Gal4 DNA activation domain (AD) fused to RhoGAP93B RhoGAP93B was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between so and RhoGAP93B was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.340269537 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 37312 CG17824 CT39558 CG17824 24663055 0 Flybase FBgn0036234 Drosophila melanogaster taxon 7227 CG17824-PA 0 cact n(2)k17003 CT18347 CG5848 dip6 cac cactus cact 24584588 0 Flybase FBgn0000250 Drosophila melanogaster taxon 7227 cactus CG5848-PA 0 CG17824 protein interacts with cact protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-CG17824 Gal4 DNA binding domain (DBD) fused to CG17824 CG17824 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-cact Gal4 DNA activation domain (AD) fused to cact cact was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG17824 and cact was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.206500608 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 28702 A3-3 EG:33C11.1 CT31841 CG11405 A3-3 20428645 0 Flybase FBgn0028550 Drosophila melanogaster taxon 7227 CG11405-PA 0 Jra l(2R)IA109 l(2)IA109 dm-Jun CT7577 CG2275 dAP-1 d-jun d-Jun d-JRA c-Jun D-jun D-Jun dJun dJra dJUN dJRA cJun Djun DJun DJUN AP-1 jun Jun AP1 Jun-related antigen Jra 24652319 0 Flybase FBgn0001291 Drosophila melanogaster taxon 7227 Jun-related antigen CG2275-PB 0 A3-3 protein interacts with Jra protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-AD-A3-3 Gal4 DNA activation domain (AD) fused to A3-3 A3-3 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-Jra Gal4 DNA binding domain (DBD) fused to Jra Jra was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between A3-3 and Jra was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.985474067 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 43740 Fmr1 EP(3)3517 CT20883 CT17036 AT24755 cg6203 CG6203 dmfr1 dfmr1 dFXR1 dFMRP dFMR1 dfxr dFXR FMR1 FXR Fragile-X-related Fmr1 24645602 0 Flybase FBgn0028734 Drosophila melanogaster taxon 7227 CG6203-PB 0 CG8854 CT25426 CG8854 24652930 0 Flybase FBgn0033702 Drosophila melanogaster taxon 7227 CG8854-PA 0 Fmr1 protein interacts with CG8854 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-Fmr1 Gal4 DNA binding domain (DBD) fused to Fmr1 Fmr1 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CG8854 Gal4 DNA activation domain (AD) fused to CG8854 CG8854 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Fmr1 and CG8854 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.090158878 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 35006 WASp D-WASP CT3925 CG1520 dWASP Wasp WASP Wsp WASp 28572033 0 Flybase FBgn0024273 Drosophila melanogaster taxon 7227 CG1520-PC 0 chic profilin l(2)27/7 Profilin CT36595 CT27020 CG9553 sand Chic chi D88 Chi chickadee chic 24582073 0 Flybase FBgn0000308 Drosophila melanogaster taxon 7227 chickadee CG9553-PC 0 WASp protein interacts with chic protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-WASp Gal4 DNA binding domain (DBD) fused to WASp WASp was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-chic Gal4 DNA activation domain (AD) fused to chic chic was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between WASp and chic was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.202802623 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 37876 ttk ttk/FTZ-F2 E(yan)100D l(3)02667 ftzf2/ttk l(3)j7B8 l(3)j2A1 CT36470 CT36468 CT36466 CG11558 1418/06 1396/14 1372/08 1325/15 1281/04 1260/10 1209/10 1209/05 1184/16 1119/04 1049/07 0702/07 0438/31 0250/25 0037/17 ttkp69 schmal ftz-f2 FTZ-F2 CT5673 CG1856 ttk69 Ttk69 TTK88 5311 5125 3540 ovs oss osn Ttk tramtrack ttk 24651713 0 Flybase FBgn0003870 Drosophila melanogaster taxon 7227 tramtrack CG1856-PC 0 CG31090 CT34269 CG14539 CG31090 24650192 0 Flybase FBgn0051090 Drosophila melanogaster taxon 7227 CG31090-PA 0 ttk protein interacts with CG31090 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-ttk Gal4 DNA activation domain (AD) fused to ttk ttk was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG31090 Gal4 DNA binding domain (DBD) fused to CG31090 CG31090 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between ttk and CG31090 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.255164657 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 37413 Cyt-c-p CT39869 CG17903 pDMc01 cyt-c2 CytC-2 Cyt-c2 cyt.c DMc01 Cytc DC4 Cytochrome c proximal Cyt-c-p 17137228 0 Flybase FBgn0000409 Drosophila melanogaster taxon 7227 Cytochrome c proximal CG17903-PA 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 Cyt-c-p protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-Cyt-c-p Gal4 DNA activation domain (AD) fused to Cyt-c-p Cyt-c-p was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Cyt-c-p and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.03664032 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 43741 Fmr1 EP(3)3517 CT20883 CT17036 AT24755 cg6203 CG6203 dmfr1 dfmr1 dFXR1 dFMRP dFMR1 dfxr dFXR FMR1 FXR Fragile-X-related Fmr1 24645602 0 Flybase FBgn0028734 Drosophila melanogaster taxon 7227 CG6203-PB 0 CG9933 20129485 0 Flybase FBgn0032468 Drosophila melanogaster taxon 7227 CG9933-PA 0 Fmr1 protein interacts with CG9933 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-DBD-Fmr1 Gal4 DNA binding domain (DBD) fused to Fmr1 Fmr1 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CG9933 Gal4 DNA activation domain (AD) fused to CG9933 CG9933 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Fmr1 and CG9933 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.651025462 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 25104 emc ms(3)61CD l(3)j4E11 l(3)05592 l(3)04322 0977/09 0587/01 0203/10 0094/26 Dm0688 CT1010 CG1007 gov Emc Ach extra macrochaetae emc 24654863 0 Flybase FBgn0000575 Drosophila melanogaster taxon 7227 extra macrochaetae CG1007-PA 0 HLH4C EG:84H4.2 DroNHLH CT10268 CG3052 Helix loop helix protein 4C HLH4C 24639715 0 Flybase FBgn0011277 Drosophila melanogaster taxon 7227 Helix loop helix protein 4C CG3052-PA 0 emc protein interacts with HLH4C protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-DBD-emc Gal4 DNA binding domain (DBD) fused to emc emc was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-HLH4C Gal4 DNA activation domain (AD) fused to HLH4C HLH4C was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between emc and HLH4C was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.846936167 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 43581 cact n(2)k17003 CT18347 CG5848 dip6 cac cactus cact 24584588 0 Flybase FBgn0000250 Drosophila melanogaster taxon 7227 cactus CG5848-PA 0 CG8925 CT25634 CG8925 24647367 0 Flybase FBgn0038404 Drosophila melanogaster taxon 7227 CG8925-PA 0 cact protein interacts with CG8925 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-cact Gal4 DNA activation domain (AD) fused to cact cact was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG8925 Gal4 DNA binding domain (DBD) fused to CG8925 CG8925 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between cact and CG8925 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.322129183 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 41961 BG:DS00929.4 BG:DS00929.4 CT13602 CG4103 19921364 0 Flybase FBgn0028925 Drosophila melanogaster taxon 7227 CG4103-PA 0 snf fs(1)A1621 fs(1)1621 snf1621 SNF/D25 CT14684 CG4528 liz Snf SNF D25 sans fille snf 17737284 0 Flybase FBgn0003449 Drosophila melanogaster taxon 7227 sans fille CG4528-PA 0 BG:DS00929.4 protein interacts with snf protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-AD-BG:DS00929.4 Gal4 DNA activation domain (AD) fused to BG:DS00929.4 BG:DS00929.4 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-snf Gal4 DNA binding domain (DBD) fused to snf snf was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between BG:DS00929.4 and snf was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.972151662 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27622 so CG11121 CT8839 somda old med mda ami Drl So sine oculis so 17136492 0 Flybase FBgn0003460 Drosophila melanogaster taxon 7227 sine oculis CG11121-PA 0 RhoGEF2 Su(Rho1)2B l(2)04291 DRhoGEF2 rhoGEF2 dRhoGEF DRhoGEF CT21257 CG9635 shar T2 RhoGEF2 24654319 0 Flybase FBgn0023172 Drosophila melanogaster taxon 7227 CG9635-PB 0 so protein interacts with RhoGEF2 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-so Gal4 DNA binding domain (DBD) fused to so so was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-RhoGEF2 Gal4 DNA activation domain (AD) fused to RhoGEF2 RhoGEF2 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between so and RhoGEF2 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.340269537 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 43582 cact n(2)k17003 CT18347 CG5848 dip6 cac cactus cact 24584588 0 Flybase FBgn0000250 Drosophila melanogaster taxon 7227 cactus CG5848-PA 0 CG9925 CT27942 CG9925 21358205 0 Flybase FBgn0038191 Drosophila melanogaster taxon 7227 CG9925-PA 0 cact protein interacts with CG9925 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-cact Gal4 DNA activation domain (AD) fused to cact cact was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG9925 Gal4 DNA binding domain (DBD) fused to CG9925 CG9925 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between cact and CG9925 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.231475203 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 36484 CG17097 CT37982 CG17097 24583487 0 Flybase FBgn0032275 Drosophila melanogaster taxon 7227 CG17097-PA 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 CG17097 protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-CG17097 Gal4 DNA activation domain (AD) fused to CG17097 CG17097 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG17097 and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.114144675 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 38291 bip2 TAFII155 bip-II CT6427 CG2009 BIP2 bip2 24638625 0 Flybase FBgn0026262 Drosophila melanogaster taxon 7227 CG2009-PA 0 Jra l(2R)IA109 l(2)IA109 dm-Jun CT7577 CG2275 dAP-1 d-jun d-Jun d-JRA c-Jun D-jun D-Jun dJun dJra dJUN dJRA cJun Djun DJun DJUN AP-1 jun Jun AP1 Jun-related antigen Jra 24652319 0 Flybase FBgn0001291 Drosophila melanogaster taxon 7227 Jun-related antigen CG2275-PB 0 bip2 protein interacts with Jra protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-bip2 Gal4 DNA activation domain (AD) fused to bip2 bip2 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-Jra Gal4 DNA binding domain (DBD) fused to Jra Jra was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between bip2 and Jra was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.088443397 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 40956 CG3308 CT11109 CG3308 24648690 0 Flybase FBgn0038877 Drosophila melanogaster taxon 7227 CG3308-PA 0 Khc l(2)k13314 l(2R)W12 l(2)W12 kinesin CT23616 CG7765 KIF5A Dmkin DmKHC kin khc Kin KHC DKH DK Kinesin heavy chain Khc 17136240 0 Flybase FBgn0001308 Drosophila melanogaster taxon 7227 Kinesin heavy chain CG7765-PA 0 CG3308 protein interacts with Khc protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-CG3308 Gal4 DNA binding domain (DBD) fused to CG3308 CG3308 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-Khc Gal4 DNA activation domain (AD) fused to Khc Khc was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG3308 and Khc was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.154623113 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 38693 Jra l(2R)IA109 l(2)IA109 dm-Jun CT7577 CG2275 dAP-1 d-jun d-Jun d-JRA c-Jun D-jun D-Jun dJun dJra dJUN dJRA cJun Djun DJun DJUN AP-1 jun Jun AP1 Jun-related antigen Jra 24652319 0 Flybase FBgn0001291 Drosophila melanogaster taxon 7227 Jun-related antigen CG2275-PB 0 Jra l(2R)IA109 l(2)IA109 dm-Jun CT7577 CG2275 dAP-1 d-jun d-Jun d-JRA c-Jun D-jun D-Jun dJun dJra dJUN dJRA cJun Djun DJun DJUN AP-1 jun Jun AP1 Jun-related antigen Jra 24652319 0 Flybase FBgn0001291 Drosophila melanogaster taxon 7227 Jun-related antigen CG2275-PB 0 Jra protein interacts with Jra protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-DBD-Jra Gal4 DNA binding domain (DBD) fused to Jra Jra was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-Jra Gal4 DNA activation domain (AD) fused to Jra Jra was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Jra and Jra was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.60633177 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 30373 Hsp83 ms(3)08445 Su(Raf)3A l(3)j5C2 E(sina)2 E(sev)3A DMHSP82 CT2354 CG1242 hsp90 hsp84 hsp83 hsp82 Hsp90 Hsp82 HSP90 HSP83 HSP82 ORF1 stc 83 Heat shock protein 83 Hsp83 17647529 0 Flybase FBgn0001233 Drosophila melanogaster taxon 7227 Heat shock protein 83 CG1242-PA 0 CG14207 CT33820 CG14207 24643312 0 Flybase FBgn0031037 Drosophila melanogaster taxon 7227 CG14207-PB 0 Hsp83 protein interacts with CG14207 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-Hsp83 Gal4 DNA activation domain (AD) fused to Hsp83 Hsp83 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG14207 Gal4 DNA binding domain (DBD) fused to CG14207 CG14207 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Hsp83 and CG14207 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.067805214 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27168 CG1091 CT1553 CG1091 28573195 0 Flybase FBgn0037470 Drosophila melanogaster taxon 7227 CG1091-PB 0 Jra l(2R)IA109 l(2)IA109 dm-Jun CT7577 CG2275 dAP-1 d-jun d-Jun d-JRA c-Jun D-jun D-Jun dJun dJra dJUN dJRA cJun Djun DJun DJUN AP-1 jun Jun AP1 Jun-related antigen Jra 24652319 0 Flybase FBgn0001291 Drosophila melanogaster taxon 7227 Jun-related antigen CG2275-PB 0 CG1091 protein interacts with Jra protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-CG1091 Gal4 DNA activation domain (AD) fused to CG1091 CG1091 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-Jra Gal4 DNA binding domain (DBD) fused to Jra Jra was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG1091 and Jra was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.167642195 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 36692 CG17265 CT35453 CG17265 24581314 0 Flybase FBgn0031488 Drosophila melanogaster taxon 7227 CG17265-PA 0 cact n(2)k17003 CT18347 CG5848 dip6 cac cactus cact 24584588 0 Flybase FBgn0000250 Drosophila melanogaster taxon 7227 cactus CG5848-PA 0 CG17265 protein interacts with cact protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-CG17265 Gal4 DNA binding domain (DBD) fused to CG17265 CG17265 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-cact Gal4 DNA activation domain (AD) fused to cact cact was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG17265 and cact was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.260655403 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27611 so CG11121 CT8839 somda old med mda ami Drl So sine oculis so 17136492 0 Flybase FBgn0003460 Drosophila melanogaster taxon 7227 sine oculis CG11121-PA 0 ab l(2)k02807 clueless CT15423 CG4807 ptd clu abrupt ab 17136198 0 Flybase FBgn0000011 Drosophila melanogaster taxon 7227 abrupt CG4807-PB 0 so protein interacts with ab protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-so Gal4 DNA binding domain (DBD) fused to so so was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-ab Gal4 DNA activation domain (AD) fused to ab ab was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between so and ab was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.183171992 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 25106 emc ms(3)61CD l(3)j4E11 l(3)05592 l(3)04322 0977/09 0587/01 0203/10 0094/26 Dm0688 CT1010 CG1007 gov Emc Ach extra macrochaetae emc 24654863 0 Flybase FBgn0000575 Drosophila melanogaster taxon 7227 extra macrochaetae CG1007-PA 0 da l(2)31Ea CT16383 CG5102 daughterless da 17137250 0 Flybase FBgn0000413 Drosophila melanogaster taxon 7227 daughterless CG5102-PA 0 emc protein interacts with da protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-emc Gal4 DNA binding domain (DBD) fused to emc emc was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-da Gal4 DNA activation domain (AD) fused to da da was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between emc and da was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.450618761 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 38695 Jra l(2R)IA109 l(2)IA109 dm-Jun CT7577 CG2275 dAP-1 d-jun d-Jun d-JRA c-Jun D-jun D-Jun dJun dJra dJUN dJRA cJun Djun DJun DJUN AP-1 jun Jun AP1 Jun-related antigen Jra 24652319 0 Flybase FBgn0001291 Drosophila melanogaster taxon 7227 Jun-related antigen CG2275-PB 0 Tm1 l(3)s2958 l(3)02299 CT15609 CT15051 TnH-34 CG4898 mTmII cTmII Tmr34 Tmr33 TmII TMII 2299 cTm cTM TnH PmI Tropomyosin 1 Tm1 24647089 0 Flybase FBgn0003721 Drosophila melanogaster taxon 7227 Tropomyosin 1 CG4898-PA 0 Jra protein interacts with Tm1 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-Jra Gal4 DNA binding domain (DBD) fused to Jra Jra was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-Tm1 Gal4 DNA activation domain (AD) fused to Tm1 Tm1 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Jra and Tm1 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.349987034 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 36406 Rst(1)JH CT4862 CG1705 Mett Met Resistance to Juvenile Hormone Rst(1)JH 24641346 0 Flybase FBgn0002723 Drosophila melanogaster taxon 7227 Resistance to Juvenile Hormone CG1705-PA 0 Khc l(2)k13314 l(2R)W12 l(2)W12 kinesin CT23616 CG7765 KIF5A Dmkin DmKHC kin khc Kin KHC DKH DK Kinesin heavy chain Khc 17136240 0 Flybase FBgn0001308 Drosophila melanogaster taxon 7227 Kinesin heavy chain CG7765-PA 0 Rst(1)JH protein interacts with Khc protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-DBD-Rst(1)JH Gal4 DNA binding domain (DBD) fused to Rst(1)JH Rst(1)JH was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-Khc Gal4 DNA activation domain (AD) fused to Khc Khc was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Rst(1)JH and Khc was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.612405635 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 38694 Jra l(2R)IA109 l(2)IA109 dm-Jun CT7577 CG2275 dAP-1 d-jun d-Jun d-JRA c-Jun D-jun D-Jun dJun dJra dJUN dJRA cJun Djun DJun DJUN AP-1 jun Jun AP1 Jun-related antigen Jra 24652319 0 Flybase FBgn0001291 Drosophila melanogaster taxon 7227 Jun-related antigen CG2275-PB 0 yin CT9924 CT9914 CG2913 opt1 opt yin yin 24639585 0 Flybase FBgn0015565 Drosophila melanogaster taxon 7227 yin CG2913-PB 0 Jra protein interacts with yin protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-AD-Jra Gal4 DNA activation domain (AD) fused to Jra Jra was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-yin Gal4 DNA binding domain (DBD) fused to yin yin was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Jra and yin was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.554602767 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 25107 emc ms(3)61CD l(3)j4E11 l(3)05592 l(3)04322 0977/09 0587/01 0203/10 0094/26 Dm0688 CT1010 CG1007 gov Emc Ach extra macrochaetae emc 24654863 0 Flybase FBgn0000575 Drosophila melanogaster taxon 7227 extra macrochaetae CG1007-PA 0 CG7849 CT5216 CG7849 24585950 0 Flybase FBgn0033060 Drosophila melanogaster taxon 7227 CG7849-PA 0 emc protein interacts with CG7849 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-emc Gal4 DNA activation domain (AD) fused to emc emc was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG7849 Gal4 DNA binding domain (DBD) fused to CG7849 CG7849 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between emc and CG7849 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.488319331 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 36434 CG17050 CT30306 CG17050 24653350 0 Flybase FBgn0033831 Drosophila melanogaster taxon 7227 CG17050-PA 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 CG17050 protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-CG17050 Gal4 DNA activation domain (AD) fused to CG17050 CG17050 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG17050 and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.089409997 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 30202 th E(rpr)3-1 Diap-1/Th l(3)j5C8 l(3)72Dc CT43237 CT18339 CG12284 1065/03 0736/01 0626/11 0604/04 0489/15 0306/05 diap-1 dIAP-1 Diap-1 DIAP-2 DIAP-1 D-iap1 D-IAP1 diap1 dIAP1 Diap1 DIAP1 dIAP Iap1 DIAP IAP thread th 24664975 0 Flybase FBgn0003691 Drosophila melanogaster taxon 7227 thread CG12284-PC 0 CG7417 CT22745 CG7417 24655577 0 Flybase FBgn0034431 Drosophila melanogaster taxon 7227 CG7417-PA 0 th protein interacts with CG7417 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-th Gal4 DNA activation domain (AD) fused to th th was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG7417 Gal4 DNA binding domain (DBD) fused to CG7417 CG7417 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between th and CG7417 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.146374235 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 28455 CG11327 CT31615 CG11327 28574613 0 Flybase FBgn0031849 Drosophila melanogaster taxon 7227 CG11327-PB 0 ttk ttk/FTZ-F2 E(yan)100D l(3)02667 ftzf2/ttk l(3)j7B8 l(3)j2A1 CT36470 CT36468 CT36466 CG11558 1418/06 1396/14 1372/08 1325/15 1281/04 1260/10 1209/10 1209/05 1184/16 1119/04 1049/07 0702/07 0438/31 0250/25 0037/17 ttkp69 schmal ftz-f2 FTZ-F2 CT5673 CG1856 ttk69 Ttk69 TTK88 5311 5125 3540 ovs oss osn Ttk tramtrack ttk 24651713 0 Flybase FBgn0003870 Drosophila melanogaster taxon 7227 tramtrack CG1856-PC 0 CG11327 protein interacts with ttk protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-CG11327 Gal4 DNA binding domain (DBD) fused to CG11327 CG11327 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-ttk Gal4 DNA activation domain (AD) fused to ttk ttk was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG11327 and ttk was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.081723297 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 43578 cact n(2)k17003 CT18347 CG5848 dip6 cac cactus cact 24584588 0 Flybase FBgn0000250 Drosophila melanogaster taxon 7227 cactus CG5848-PA 0 Cyp6t3 24655751 0 Flybase FBgn0034447 Drosophila melanogaster taxon 7227 CG7744-PA or CG8457-PA This is a modelled interaction record. The cDNA isolated for this molecule was mapped to a cluster containing multiple genes (Table S2, Supplementary Methods and Materials). This cluster contains the following genes: CG7744 or CG8457 . 0 cact protein interacts with Cyp6t3 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). This is a modelled interaction record. Molecule B was mapped to a cluster containing multiple genes (Table S2, Supplementary Methods and Materials). 0 0 34 Gal4-AD-cact Gal4 DNA activation domain (AD) fused to cact cact was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-Cyp6t3 Gal4 DNA binding domain (DBD) fused to Cyp6t3 Cyp6t3 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. The cDNA isolated for this molecule was mapped to a cluster containing multiple genes (Table S2, Supplementary Methods and Materials). This cluster contains the following genes: CG7744, CG8457 0 An interaction between cact and Cyp6t3 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.260701054 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 30203 th E(rpr)3-1 Diap-1/Th l(3)j5C8 l(3)72Dc CT43237 CT18339 CG12284 1065/03 0736/01 0626/11 0604/04 0489/15 0306/05 diap-1 dIAP-1 Diap-1 DIAP-2 DIAP-1 D-iap1 D-IAP1 diap1 dIAP1 Diap1 DIAP1 dIAP Iap1 DIAP IAP thread th 24664975 0 Flybase FBgn0003691 Drosophila melanogaster taxon 7227 thread CG12284-PC 0 sina CT28005 CG9949 sia seven in absentia sina 24665579 0 Flybase FBgn0003410 Drosophila melanogaster taxon 7227 seven in absentia CG9949-PB 0 th protein interacts with sina protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-th Gal4 DNA activation domain (AD) fused to th th was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-sina Gal4 DNA binding domain (DBD) fused to sina sina was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between th and sina was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.264119501 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 43988 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 CG6707 CT37151 CT20842 CG6707 24662056 0 Flybase FBgn0036058 Drosophila melanogaster taxon 7227 CG6707-PB 0 dl protein interacts with CG6707 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CG6707 Gal4 DNA activation domain (AD) fused to CG6707 CG6707 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between dl and CG6707 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.065568389 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 43020 CG5174 CT39031 CG5174 24655036 0 Flybase FBgn0034345 Drosophila melanogaster taxon 7227 CG5174-PB 0 ash1 l(3)SG29a CT25366 CG8887 ash-1 dash Ash1 ASH1 ash absent, small, or homeotic discs 1 ash1 17737643 0 Flybase FBgn0005386 Drosophila melanogaster taxon 7227 absent, small, or homeotic discs 1 CG8887-PA 0 CG5174 protein interacts with ash1 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-CG5174 Gal4 DNA binding domain (DBD) fused to CG5174 CG5174 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-ash1 Gal4 DNA activation domain (AD) fused to ash1 ash1 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG5174 and ash1 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.377930189 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 43579 cact n(2)k17003 CT18347 CG5848 dip6 cac cactus cact 24584588 0 Flybase FBgn0000250 Drosophila melanogaster taxon 7227 cactus CG5848-PA 0 CG7856 CT3630 CG7856 24585943 0 Flybase FBgn0033056 Drosophila melanogaster taxon 7227 CG7856-PA 0 cact protein interacts with CG7856 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-cact Gal4 DNA activation domain (AD) fused to cact cact was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG7856 Gal4 DNA binding domain (DBD) fused to CG7856 CG7856 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between cact and CG7856 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.054340609 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 28775 CG11455 CT36251 CG11455 24580574 0 Flybase FBgn0031228 Drosophila melanogaster taxon 7227 CG11455-PB 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 CG11455 protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-CG11455 Gal4 DNA activation domain (AD) fused to CG11455 CG11455 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG11455 and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.138121378 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 42783 RpP1 bs34h02.y1 CT15790 CG4918 rpA1 RpA1 Tp1 T1 Ribosomal protein P1 RpP1 17647887 0 Flybase FBgn0003274 Drosophila melanogaster taxon 7227 Ribosomal protein P1 CG4918-PA 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 RpP1 protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-RpP1 Gal4 DNA activation domain (AD) fused to RpP1 RpP1 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between RpP1 and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.045166891 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 43573 cact n(2)k17003 CT18347 CG5848 dip6 cac cactus cact 24584588 0 Flybase FBgn0000250 Drosophila melanogaster taxon 7227 cactus CG5848-PA 0 Fmr1 EP(3)3517 CT20883 CT17036 AT24755 cg6203 CG6203 dmfr1 dfmr1 dFXR1 dFMRP dFMR1 dfxr dFXR FMR1 FXR Fragile-X-related Fmr1 24645602 0 Flybase FBgn0028734 Drosophila melanogaster taxon 7227 CG6203-PB 0 cact protein interacts with Fmr1 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-AD-cact Gal4 DNA activation domain (AD) fused to cact cact was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-Fmr1 Gal4 DNA binding domain (DBD) fused to Fmr1 Fmr1 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between cact and Fmr1 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.667861055 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27891 CG11166 CT31190 CT4870 CG1706 CG11166 24586220 0 Flybase FBgn0033166 Drosophila melanogaster taxon 7227 CG11166-PB 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 CG11166 protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-CG11166 Gal4 DNA activation domain (AD) fused to CG11166 CG11166 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG11166 and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.178935037 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 37875 ttk ttk/FTZ-F2 E(yan)100D l(3)02667 ftzf2/ttk l(3)j7B8 l(3)j2A1 CT36470 CT36468 CT36466 CG11558 1418/06 1396/14 1372/08 1325/15 1281/04 1260/10 1209/10 1209/05 1184/16 1119/04 1049/07 0702/07 0438/31 0250/25 0037/17 ttkp69 schmal ftz-f2 FTZ-F2 CT5673 CG1856 ttk69 Ttk69 TTK88 5311 5125 3540 ovs oss osn Ttk tramtrack ttk 24651713 0 Flybase FBgn0003870 Drosophila melanogaster taxon 7227 tramtrack CG1856-PC 0 lwr l(2)05487 l(2)05486 l(2)02858 l(2)01519 CT36973 Dmubc9 DmUbc9 CG3018 dUbc9 DUbc9 ubc9 semi dip4 Ubc9 UBC9 hbl Lwr lesswright lwr 24580658 0 Flybase FBgn0010602 Drosophila melanogaster taxon 7227 lesswright CG3018-PB 0 ttk protein interacts with lwr protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-ttk Gal4 DNA binding domain (DBD) fused to ttk ttk was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-lwr Gal4 DNA activation domain (AD) fused to lwr lwr was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between ttk and lwr was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.164353923 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27384 FK506-bp2 FK506-binding protein DrFKBP12 dFKBP12 CT30803 CG11001 FKBP12 FKBP FK506-binding protein 2 FK506-bp2 24655568 0 Flybase FBgn0013954 Drosophila melanogaster taxon 7227 FK506-binding protein 2 CG11001-PA 0 csw l(1)GA114 l(1)G0170 E(sev)1A Csw/Shp2 l(1)csw l(1)2Dd l(1)2Db CT43319 CT37554 CT13063 CG3954 19-106 Csw CSW corkscrew csw 24639268 0 Flybase FBgn0000382 Drosophila melanogaster taxon 7227 corkscrew CG3954-PC 0 FK506-bp2 protein interacts with csw protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-FK506-bp2 Gal4 DNA activation domain (AD) fused to FK506-bp2 FK506-bp2 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-csw Gal4 DNA binding domain (DBD) fused to csw csw was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between FK506-bp2 and csw was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.325419713 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 43233 Klc CT17238 CG5433 DKLC klc KLC Kinesin light chain Klc 17647555 0 Flybase FBgn0010235 Drosophila melanogaster taxon 7227 Kinesin light chain CG5433-PA 0 Khc l(2)k13314 l(2R)W12 l(2)W12 kinesin CT23616 CG7765 KIF5A Dmkin DmKHC kin khc Kin KHC DKH DK Kinesin heavy chain Khc 17136240 0 Flybase FBgn0001308 Drosophila melanogaster taxon 7227 Kinesin heavy chain CG7765-PA 0 Klc protein interacts with Khc protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-DBD-Klc Gal4 DNA binding domain (DBD) fused to Klc Klc was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-Khc Gal4 DNA activation domain (AD) fused to Khc Khc was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Klc and Khc was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.618248326 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 25682 D19A CT28851 CG10269 D19A 17352479 0 Flybase FBgn0022935 Drosophila melanogaster taxon 7227 CG10269-PA 0 cact n(2)k17003 CT18347 CG5848 dip6 cac cactus cact 24584588 0 Flybase FBgn0000250 Drosophila melanogaster taxon 7227 cactus CG5848-PA 0 D19A protein interacts with cact protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-D19A Gal4 DNA binding domain (DBD) fused to D19A D19A was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-cact Gal4 DNA activation domain (AD) fused to cact cact was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between D19A and cact was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.490345687 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 35384 kay CT35622 CG15509 dAP-1 d-fos c-Fos D-fos D-Fos dlhD dFra dFos dFRA Dfos DFra DFos DFOS AP-1 sro fos Fra Fos AP1 kayak kay 24651137 0 Flybase FBgn0001297 Drosophila melanogaster taxon 7227 kayak CG15509-PB 0 Jra l(2R)IA109 l(2)IA109 dm-Jun CT7577 CG2275 dAP-1 d-jun d-Jun d-JRA c-Jun D-jun D-Jun dJun dJra dJUN dJRA cJun Djun DJun DJUN AP-1 jun Jun AP1 Jun-related antigen Jra 24652319 0 Flybase FBgn0001291 Drosophila melanogaster taxon 7227 Jun-related antigen CG2275-PB 0 kay protein interacts with Jra protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-AD-kay Gal4 DNA activation domain (AD) fused to kay kay was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-Jra Gal4 DNA binding domain (DBD) fused to Jra Jra was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between kay and Jra was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.591725838 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 37878 ttk ttk/FTZ-F2 E(yan)100D l(3)02667 ftzf2/ttk l(3)j7B8 l(3)j2A1 CT36470 CT36468 CT36466 CG11558 1418/06 1396/14 1372/08 1325/15 1281/04 1260/10 1209/10 1209/05 1184/16 1119/04 1049/07 0702/07 0438/31 0250/25 0037/17 ttkp69 schmal ftz-f2 FTZ-F2 CT5673 CG1856 ttk69 Ttk69 TTK88 5311 5125 3540 ovs oss osn Ttk tramtrack ttk 24651713 0 Flybase FBgn0003870 Drosophila melanogaster taxon 7227 tramtrack CG1856-PC 0 CG31365 CT14997 CG4639 CG31365 24649219 0 Flybase FBgn0051365 Drosophila melanogaster taxon 7227 CG31365-PA 0 ttk protein interacts with CG31365 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-ttk Gal4 DNA binding domain (DBD) fused to ttk ttk was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CG31365 Gal4 DNA activation domain (AD) fused to CG31365 CG31365 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between ttk and CG31365 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.253646814 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 33920 CG14546 CT34276 CG14546 24650202 0 Flybase FBgn0039395 Drosophila melanogaster taxon 7227 CG14546-PA 0 ttk ttk/FTZ-F2 E(yan)100D l(3)02667 ftzf2/ttk l(3)j7B8 l(3)j2A1 CT36470 CT36468 CT36466 CG11558 1418/06 1396/14 1372/08 1325/15 1281/04 1260/10 1209/10 1209/05 1184/16 1119/04 1049/07 0702/07 0438/31 0250/25 0037/17 ttkp69 schmal ftz-f2 FTZ-F2 CT5673 CG1856 ttk69 Ttk69 TTK88 5311 5125 3540 ovs oss osn Ttk tramtrack ttk 24651713 0 Flybase FBgn0003870 Drosophila melanogaster taxon 7227 tramtrack CG1856-PC 0 CG14546 protein interacts with ttk protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-DBD-CG14546 Gal4 DNA binding domain (DBD) fused to CG14546 CG14546 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-ttk Gal4 DNA activation domain (AD) fused to ttk ttk was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG14546 and ttk was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.780257302 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 37881 ttk ttk/FTZ-F2 E(yan)100D l(3)02667 ftzf2/ttk l(3)j7B8 l(3)j2A1 CT36470 CT36468 CT36466 CG11558 1418/06 1396/14 1372/08 1325/15 1281/04 1260/10 1209/10 1209/05 1184/16 1119/04 1049/07 0702/07 0438/31 0250/25 0037/17 ttkp69 schmal ftz-f2 FTZ-F2 CT5673 CG1856 ttk69 Ttk69 TTK88 5311 5125 3540 ovs oss osn Ttk tramtrack ttk 24651713 0 Flybase FBgn0003870 Drosophila melanogaster taxon 7227 tramtrack CG1856-PC 0 Stat92E l(3)06346 l(3)j6C8 Dstat92E stat92E marelle SD-stat DRODSRC CT13948 d-STAT D-stat D-Stat D-STAT CG4257 dSTAT Dstat DSTAT stat Stat STAT DSRC mrl Signal-transducer and activator of transcription protein at 92E Stat92E 28572156 0 Flybase FBgn0016917 Drosophila melanogaster taxon 7227 Signal-transducer and activator of transcription protein at 92E CG4257-PD 0 ttk protein interacts with Stat92E protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-ttk Gal4 DNA binding domain (DBD) fused to ttk ttk was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-Stat92E Gal4 DNA activation domain (AD) fused to Stat92E Stat92E was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between ttk and Stat92E was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.310711559 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 38714 Ccp84Ad CT7798 CG2341 cc5 Ccp84Ad 21358131 0 Flybase FBgn0004780 Drosophila melanogaster taxon 7227 CG2341-PA 0 chic profilin l(2)27/7 Profilin CT36595 CT27020 CG9553 sand Chic chi D88 Chi chickadee chic 24582073 0 Flybase FBgn0000308 Drosophila melanogaster taxon 7227 chickadee CG9553-PC 0 Ccp84Ad protein interacts with chic protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-Ccp84Ad Gal4 DNA binding domain (DBD) fused to Ccp84Ad Ccp84Ad was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-chic Gal4 DNA activation domain (AD) fused to chic chic was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Ccp84Ad and chic was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.297306977 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 29632 CG11885 CT33132 CG11885 20129129 0 Flybase FBgn0031253 Drosophila melanogaster taxon 7227 CG11885-PA 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 CG11885 protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-CG11885 Gal4 DNA activation domain (AD) fused to CG11885 CG11885 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG11885 and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.178935037 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 43580 cact n(2)k17003 CT18347 CG5848 dip6 cac cactus cact 24584588 0 Flybase FBgn0000250 Drosophila melanogaster taxon 7227 cactus CG5848-PA 0 CG8417 CT24707 CG8417 21356061 0 Flybase FBgn0037744 Drosophila melanogaster taxon 7227 CG8417-PA 0 cact protein interacts with CG8417 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-cact Gal4 DNA activation domain (AD) fused to cact cact was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG8417 Gal4 DNA binding domain (DBD) fused to CG8417 CG8417 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between cact and CG8417 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.086809634 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 32040 CG13321 CT32637 CG13321 19922144 0 Flybase FBgn0033787 Drosophila melanogaster taxon 7227 CG13321-PA 0 Khc l(2)k13314 l(2R)W12 l(2)W12 kinesin CT23616 CG7765 KIF5A Dmkin DmKHC kin khc Kin KHC DKH DK Kinesin heavy chain Khc 17136240 0 Flybase FBgn0001308 Drosophila melanogaster taxon 7227 Kinesin heavy chain CG7765-PA 0 CG13321 protein interacts with Khc protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-CG13321 Gal4 DNA binding domain (DBD) fused to CG13321 CG13321 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-Khc Gal4 DNA activation domain (AD) fused to Khc Khc was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG13321 and Khc was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.2596675 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 29193 Abd-B Uab-5[Sab] l(3)89Ed DROABDB CT40560 CT36635 CT28893 CG11648 CG10291 AbdB-II iab8.9 AbdB-I tuh-3 pH189 iab-8 iab-7 iab-6 iab-5 abd-B Fab-7 Abd-b twig iab9 iab8 iab7 iab6 iab5 abdB Fab7 AbdB ABDB iab Tab Sab Mcp MCP FAB Mc Abdominal B Abd-B 24647546 0 Flybase FBgn0000015 Drosophila melanogaster taxon 7227 Abdominal B CG11648-PD 0 CG13728 CT33195 CG13728 28574876 0 Flybase FBgn0036716 Drosophila melanogaster taxon 7227 CG13728-PA 0 Abd-B protein interacts with CG13728 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-Abd-B Gal4 DNA binding domain (DBD) fused to Abd-B Abd-B was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CG13728 Gal4 DNA activation domain (AD) fused to CG13728 CG13728 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Abd-B and CG13728 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.254088756 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 37874 ttk ttk/FTZ-F2 E(yan)100D l(3)02667 ftzf2/ttk l(3)j7B8 l(3)j2A1 CT36470 CT36468 CT36466 CG11558 1418/06 1396/14 1372/08 1325/15 1281/04 1260/10 1209/10 1209/05 1184/16 1119/04 1049/07 0702/07 0438/31 0250/25 0037/17 ttkp69 schmal ftz-f2 FTZ-F2 CT5673 CG1856 ttk69 Ttk69 TTK88 5311 5125 3540 ovs oss osn Ttk tramtrack ttk 24651713 0 Flybase FBgn0003870 Drosophila melanogaster taxon 7227 tramtrack CG1856-PC 0 bip2 TAFII155 bip-II CT6427 CG2009 BIP2 bip2 24638625 0 Flybase FBgn0026262 Drosophila melanogaster taxon 7227 CG2009-PA 0 ttk protein interacts with bip2 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-ttk Gal4 DNA binding domain (DBD) fused to ttk ttk was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-bip2 Gal4 DNA activation domain (AD) fused to bip2 bip2 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between ttk and bip2 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.16104129 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 33409 CG14204 CT33817 CG14204 24643303 0 Flybase FBgn0031032 Drosophila melanogaster taxon 7227 CG14204-PA 0 cact n(2)k17003 CT18347 CG5848 dip6 cac cactus cact 24584588 0 Flybase FBgn0000250 Drosophila melanogaster taxon 7227 cactus CG5848-PA 0 CG14204 protein interacts with cact protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-DBD-CG14204 Gal4 DNA binding domain (DBD) fused to CG14204 CG14204 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-cact Gal4 DNA activation domain (AD) fused to cact cact was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG14204 and cact was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.938474165 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 37879 ttk ttk/FTZ-F2 E(yan)100D l(3)02667 ftzf2/ttk l(3)j7B8 l(3)j2A1 CT36470 CT36468 CT36466 CG11558 1418/06 1396/14 1372/08 1325/15 1281/04 1260/10 1209/10 1209/05 1184/16 1119/04 1049/07 0702/07 0438/31 0250/25 0037/17 ttkp69 schmal ftz-f2 FTZ-F2 CT5673 CG1856 ttk69 Ttk69 TTK88 5311 5125 3540 ovs oss osn Ttk tramtrack ttk 24651713 0 Flybase FBgn0003870 Drosophila melanogaster taxon 7227 tramtrack CG1856-PC 0 Pp4-19C CG32505 CG18339 CT3537 CT3517 CG1596 CG1459 pp4 PpX PPX PP4 Protein phosphatase 19C Pp4-19C 28571314 0 Flybase FBgn0023177 Drosophila melanogaster taxon 7227 Protein phosphatase 19C CG32505-PF 0 ttk protein interacts with Pp4-19C protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-ttk Gal4 DNA binding domain (DBD) fused to ttk ttk was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-Pp4-19C Gal4 DNA activation domain (AD) fused to Pp4-19C Pp4-19C was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between ttk and Pp4-19C was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.31146626 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 25853 abd-A l(3)89Ec CT29034 CT28977 CG10325 DmabdA iab-5 iab-4 iab-3 iab-2 abd-a Abd-A iab4 iab3 iab2 abda abdA Cbxd Abda AbdA iab Hab abdominal A abd-A 24647534 0 Flybase FBgn0000014 Drosophila melanogaster taxon 7227 abdominal A CG10325-PB 0 CycG CT36413 CT36409 CT36405 CT35376 CG11525 dCycG Cyclin G CycG 24651679 0 Flybase FBgn0039858 Drosophila melanogaster taxon 7227 Cyclin G CG11525-PE 0 abd-A protein interacts with CycG protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-abd-A Gal4 DNA activation domain (AD) fused to abd-A abd-A was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CycG Gal4 DNA binding domain (DBD) fused to CycG CycG was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between abd-A and CycG was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.40987592 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 37877 ttk ttk/FTZ-F2 E(yan)100D l(3)02667 ftzf2/ttk l(3)j7B8 l(3)j2A1 CT36470 CT36468 CT36466 CG11558 1418/06 1396/14 1372/08 1325/15 1281/04 1260/10 1209/10 1209/05 1184/16 1119/04 1049/07 0702/07 0438/31 0250/25 0037/17 ttkp69 schmal ftz-f2 FTZ-F2 CT5673 CG1856 ttk69 Ttk69 TTK88 5311 5125 3540 ovs oss osn Ttk tramtrack ttk 24651713 0 Flybase FBgn0003870 Drosophila melanogaster taxon 7227 tramtrack CG1856-PC 0 CCR4 CT39331 CT33003 CT17512 CG17741 CG5534 CCR4 28572072 0 Flybase FBgn0039168 Drosophila melanogaster taxon 7227 CG31137-PF 0 ttk protein interacts with CCR4 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-ttk Gal4 DNA binding domain (DBD) fused to ttk ttk was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CCR4 Gal4 DNA activation domain (AD) fused to CCR4 CCR4 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between ttk and CCR4 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.248954331 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 44459 Arp66B CT23123 Actr66B AcTr66B arp66B CG7558 arp3 Arp3 ARP3 Actin-related protein 66B Arp66B 17737543 0 Flybase FBgn0011744 Drosophila melanogaster taxon 7227 Actin-related protein 66B CG7558-PA 0 chic profilin l(2)27/7 Profilin CT36595 CT27020 CG9553 sand Chic chi D88 Chi chickadee chic 24582073 0 Flybase FBgn0000308 Drosophila melanogaster taxon 7227 chickadee CG9553-PC 0 Arp66B protein interacts with chic protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-Arp66B Gal4 DNA binding domain (DBD) fused to Arp66B Arp66B was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-chic Gal4 DNA activation domain (AD) fused to chic chic was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Arp66B and chic was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.266937017 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 43504 CG5733 CT18026 CG5733 24654843 0 Flybase FBgn0034310 Drosophila melanogaster taxon 7227 CG5733-PA 0 cact n(2)k17003 CT18347 CG5848 dip6 cac cactus cact 24584588 0 Flybase FBgn0000250 Drosophila melanogaster taxon 7227 cactus CG5848-PA 0 CG5733 protein interacts with cact protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-CG5733 Gal4 DNA binding domain (DBD) fused to CG5733 CG5733 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-cact Gal4 DNA activation domain (AD) fused to cact cact was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG5733 and cact was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.312504058 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27577 dsx CT39510 CT29316 CG11094 ix-62c Dmdsx Hr doublesex dsx 28573186 0 Flybase FBgn0000504 Drosophila melanogaster taxon 7227 doublesex CG11094-PC 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 dsx protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-dsx Gal4 DNA activation domain (AD) fused to dsx dsx was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between dsx and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.055406267 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27667 CG11138 CT31139 CG11138 24641557 0 Flybase FBgn0030400 Drosophila melanogaster taxon 7227 CG11138-PB 0 ttk ttk/FTZ-F2 E(yan)100D l(3)02667 ftzf2/ttk l(3)j7B8 l(3)j2A1 CT36470 CT36468 CT36466 CG11558 1418/06 1396/14 1372/08 1325/15 1281/04 1260/10 1209/10 1209/05 1184/16 1119/04 1049/07 0702/07 0438/31 0250/25 0037/17 ttkp69 schmal ftz-f2 FTZ-F2 CT5673 CG1856 ttk69 Ttk69 TTK88 5311 5125 3540 ovs oss osn Ttk tramtrack ttk 24651713 0 Flybase FBgn0003870 Drosophila melanogaster taxon 7227 tramtrack CG1856-PC 0 CG11138 protein interacts with ttk protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-CG11138 Gal4 DNA activation domain (AD) fused to CG11138 CG11138 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-ttk Gal4 DNA binding domain (DBD) fused to ttk ttk was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG11138 and ttk was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.035368869 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27154 osk CT41904 CT30527 CG10901 oskar osk 24645205 0 Flybase FBgn0003015 Drosophila melanogaster taxon 7227 oskar CG10901-PA 0 CG5383 CT17088 CG5383 21355815 0 Flybase FBgn0038948 Drosophila melanogaster taxon 7227 CG5383-PA 0 osk protein interacts with CG5383 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-osk Gal4 DNA activation domain (AD) fused to osk osk was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG5383 Gal4 DNA binding domain (DBD) fused to CG5383 CG5383 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between osk and CG5383 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.227295649 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 30375 Hsp83 ms(3)08445 Su(Raf)3A l(3)j5C2 E(sina)2 E(sev)3A DMHSP82 CT2354 CG1242 hsp90 hsp84 hsp83 hsp82 Hsp90 Hsp82 HSP90 HSP83 HSP82 ORF1 stc 83 Heat shock protein 83 Hsp83 17647529 0 Flybase FBgn0001233 Drosophila melanogaster taxon 7227 Heat shock protein 83 CG1242-PA 0 CG15308 CT35285 CG15308 24640973 0 Flybase FBgn0040941 Drosophila melanogaster taxon 7227 CG15308-PA 0 Hsp83 protein interacts with CG15308 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-Hsp83 Gal4 DNA activation domain (AD) fused to Hsp83 Hsp83 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG15308 Gal4 DNA binding domain (DBD) fused to CG15308 CG15308 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Hsp83 and CG15308 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.066426998 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 36091 guf ornithine decarboxylase antizyme l(2)k09037 l(2)90/37 l(2)154/1 l(2)131/7 l(2)02833 CT41667 CT41665 CT41663 CT37253 CG16747 guf ODA AZ Ornithine decarboxylase antizyme Oda 24652899 0 Flybase FBgn0014184 Drosophila melanogaster taxon 7227 gut feeling CG16747-PB 0 ci l(4)102ABc l(4)17 l(4)13 CT6641 CG2125 ci[D] Ci[D] ci-D ciD CiD CID Ci Ce cubitus interruptus ci 24638496 0 Flybase FBgn0004859 Drosophila melanogaster taxon 7227 cubitus interruptus CG2125-PA 0 guf protein interacts with ci protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-guf Gal4 DNA binding domain (DBD) fused to guf guf was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-ci Gal4 DNA activation domain (AD) fused to ci ci was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between guf and ci was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.21681689 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 25793 RpS26 l(2)04553 CT28945 CG10305 rpS31 rpS26 Ribosomal protein S26 RpS26 24584966 0 Flybase FBgn0004413 Drosophila melanogaster taxon 7227 Ribosomal protein S26 CG10305-PC 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 RpS26 protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-RpS26 Gal4 DNA activation domain (AD) fused to RpS26 RpS26 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between RpS26 and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.097210569 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27155 osk CT41904 CT30527 CG10901 oskar osk 24645205 0 Flybase FBgn0003015 Drosophila melanogaster taxon 7227 oskar CG10901-PA 0 CG8388 CT18261 CG8388 20130027 0 Flybase FBgn0034062 Drosophila melanogaster taxon 7227 CG8388-PA 0 osk protein interacts with CG8388 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-osk Gal4 DNA activation domain (AD) fused to osk osk was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG8388 Gal4 DNA binding domain (DBD) fused to CG8388 CG8388 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between osk and CG8388 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.487543077 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 30376 Hsp83 ms(3)08445 Su(Raf)3A l(3)j5C2 E(sina)2 E(sev)3A DMHSP82 CT2354 CG1242 hsp90 hsp84 hsp83 hsp82 Hsp90 Hsp82 HSP90 HSP83 HSP82 ORF1 stc 83 Heat shock protein 83 Hsp83 17647529 0 Flybase FBgn0001233 Drosophila melanogaster taxon 7227 Heat shock protein 83 CG1242-PA 0 BEST:LD13681 BEST:LD13681 LD13681 CT15001 CG16833 Drosophila melanogaster taxon 7227 CG16833-PC The cDNA isolated for this molecule contained only 3' untranslated sequence corresponding to GI 24583598 (Table S7, Supplementary Table). 0 Hsp83 protein interacts with BEST:LD13681 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). The cDNA isolated for BEST:LD13681 contained only 3' untranslated sequence (Table S7, Supplementary Table). 0 0 34 Gal4-AD-Hsp83 Gal4 DNA activation domain (AD) fused to Hsp83 Hsp83 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-BEST:LD13681 Gal4 DNA binding domain (DBD) fused to BEST:LD13681 BEST:LD13681 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. The cDNA isolated for BEST:LD13681 contained only 3' untranslated sequence (Table S7, Supplementary Table) 0 An interaction between Hsp83 and BEST:LD13681 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.076021059 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 28260 CG11275 CT31473 CG11275 24657782 0 Flybase FBgn0034706 Drosophila melanogaster taxon 7227 CG11275-PA 0 pnt pointed-P2 pointed-P1 pointedP2 l(3)07825 DMPOINT1A l(3)j1B7 E(E2F)3D Ets58AB D-ets-2 D-Ets-2 CT37466 CG17077 0998/12 0608/07 0123/09 ets94F Ets94F POINT EY3-1 EK3-2 Ets2 ETS2 3520 ptd pointed pnt 24649295 0 Flybase FBgn0003118 Drosophila melanogaster taxon 7227 pointed CG17077-PC 0 CG11275 protein interacts with pnt protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-DBD-CG11275 Gal4 DNA binding domain (DBD) fused to CG11275 CG11275 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-pnt Gal4 DNA activation domain (AD) fused to pnt pnt was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG11275 and pnt was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.932070359 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 33609 CG14317 CT33947 CG14317 24647823 0 Flybase FBgn0038566 Drosophila melanogaster taxon 7227 CG14317-PA 0 ttk ttk/FTZ-F2 E(yan)100D l(3)02667 ftzf2/ttk l(3)j7B8 l(3)j2A1 CT36470 CT36468 CT36466 CG11558 1418/06 1396/14 1372/08 1325/15 1281/04 1260/10 1209/10 1209/05 1184/16 1119/04 1049/07 0702/07 0438/31 0250/25 0037/17 ttkp69 schmal ftz-f2 FTZ-F2 CT5673 CG1856 ttk69 Ttk69 TTK88 5311 5125 3540 ovs oss osn Ttk tramtrack ttk 24651713 0 Flybase FBgn0003870 Drosophila melanogaster taxon 7227 tramtrack CG1856-PC 0 CG14317 protein interacts with ttk protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-CG14317 Gal4 DNA activation domain (AD) fused to CG14317 CG14317 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-ttk Gal4 DNA binding domain (DBD) fused to ttk ttk was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG14317 and ttk was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.367090021 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 32347 CG13474 CT32839 CG13474 24664257 0 Flybase FBgn0036439 Drosophila melanogaster taxon 7227 CG13474-PA 0 Khc l(2)k13314 l(2R)W12 l(2)W12 kinesin CT23616 CG7765 KIF5A Dmkin DmKHC kin khc Kin KHC DKH DK Kinesin heavy chain Khc 17136240 0 Flybase FBgn0001308 Drosophila melanogaster taxon 7227 Kinesin heavy chain CG7765-PA 0 CG13474 protein interacts with Khc protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-CG13474 Gal4 DNA binding domain (DBD) fused to CG13474 CG13474 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-Khc Gal4 DNA activation domain (AD) fused to Khc Khc was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG13474 and Khc was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.176829611 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 35836 CG15781 CT36045 CG15781 24639885 0 Flybase FBgn0029774 Drosophila melanogaster taxon 7227 CG15781-PA 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 CG15781 protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-CG15781 Gal4 DNA activation domain (AD) fused to CG15781 CG15781 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG15781 and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.125681951 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 29032 arm beta-catenin l(1)G0234 l(1)G0192 b-catenin EG:86E4.6 l(1)arm l(1)2Bv catenin CT36522 CT36518 CT12773 CG11579 Arm ARM armadillo arm 24639206 0 Flybase FBgn0000117 Drosophila melanogaster taxon 7227 armadillo CG11579-PC 0 CG14667 CT34449 CG14667 24644214 0 Flybase FBgn0037317 Drosophila melanogaster taxon 7227 CG14667-PA 0 arm protein interacts with CG14667 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-arm Gal4 DNA activation domain (AD) fused to arm arm was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG14667 Gal4 DNA binding domain (DBD) fused to CG14667 CG14667 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between arm and CG14667 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.159822064 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 40122 Trx-2 dmtrx-2 CT12877 CG31884 CG3864 thioredoxin-2 Trx-2 24583090 0 Flybase FBgn0040070 Drosophila melanogaster taxon 7227 thioredoxin-2 CG31884-PB 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 Trx-2 protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-Trx-2 Gal4 DNA activation domain (AD) fused to Trx-2 Trx-2 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Trx-2 and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.07698245 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 43992 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 Spn4 CT42827 CT42825 CT26788 CG9453 sp4 Sp4 Serine protease inhibitor 4 Spn4 Drosophila melanogaster taxon 7227 Serine protease inhibitor 4 CG9453-PA The cDNA isolated for this molecule contained only 3' untranslated sequence corresponding to GI 24586107 (Table S7, Supplementary Table). 0 dl protein interacts with Spn4 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). The cDNA isolated for Spn4 contained only 3' untranslated sequence (Table S7, Supplementary Table). 0 0 34 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-Spn4 Gal4 DNA activation domain (AD) fused to Spn4 Spn4 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. The cDNA isolated for Spn4 contained only 3' untranslated sequence (Table S7, Supplementary Table) 0 An interaction between dl and Spn4 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.07698245 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 31384 CG13030 CT32248 CG13030 24665583 0 Flybase FBgn0036669 Drosophila melanogaster taxon 7227 CG13030-PA 0 ttk ttk/FTZ-F2 E(yan)100D l(3)02667 ftzf2/ttk l(3)j7B8 l(3)j2A1 CT36470 CT36468 CT36466 CG11558 1418/06 1396/14 1372/08 1325/15 1281/04 1260/10 1209/10 1209/05 1184/16 1119/04 1049/07 0702/07 0438/31 0250/25 0037/17 ttkp69 schmal ftz-f2 FTZ-F2 CT5673 CG1856 ttk69 Ttk69 TTK88 5311 5125 3540 ovs oss osn Ttk tramtrack ttk 24651713 0 Flybase FBgn0003870 Drosophila melanogaster taxon 7227 tramtrack CG1856-PC 0 CG13030 protein interacts with ttk protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-AD-CG13030 Gal4 DNA activation domain (AD) fused to CG13030 CG13030 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-ttk Gal4 DNA binding domain (DBD) fused to ttk ttk was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG13030 and ttk was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.939089502 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 41879 CG4046 CT13148 CG4046 19922746 0 Flybase FBgn0034743 Drosophila melanogaster taxon 7227 CG4046-PA 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 CG4046 protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-CG4046 Gal4 DNA activation domain (AD) fused to CG4046 CG4046 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG4046 and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.053050744 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 31259 CG12963 CT32155 CG12963 24653890 0 Flybase FBgn0034031 Drosophila melanogaster taxon 7227 CG12963-PB 0 csw l(1)GA114 l(1)G0170 E(sev)1A Csw/Shp2 l(1)csw l(1)2Dd l(1)2Db CT43319 CT37554 CT13063 CG3954 19-106 Csw CSW corkscrew csw 24639268 0 Flybase FBgn0000382 Drosophila melanogaster taxon 7227 corkscrew CG3954-PC 0 CG12963 protein interacts with csw protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-AD-CG12963 Gal4 DNA activation domain (AD) fused to CG12963 CG12963 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-csw Gal4 DNA binding domain (DBD) fused to csw csw was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG12963 and csw was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.671806636 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 43989 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 CG6735 CT20566 CG6735 24664434 0 Flybase FBgn0036472 Drosophila melanogaster taxon 7227 CG6735-PA 0 dl protein interacts with CG6735 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CG6735 Gal4 DNA activation domain (AD) fused to CG6735 CG6735 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between dl and CG6735 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.02402689 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 43082 RpS27A l(2)04820 mfs(2)48 mfs(2)31 l(2)31Ei Ubi-f80 CT16829 CG5271 mfs48 Ubi-m UB3-D Dub80 DUb80 Ribosomal protein S27A RpS27A 17136574 0 Flybase FBgn0003942 Drosophila melanogaster taxon 7227 Ribosomal protein S27A CG5271-PA 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 RpS27A protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-RpS27A Gal4 DNA activation domain (AD) fused to RpS27A RpS27A was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between RpS27A and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.052624147 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 25234 tra2 CT28507 CG10128 tra-2 TRA-2 Tra2 transformer 2 tra2 19550175 0 Flybase FBgn0003742 Drosophila melanogaster taxon 7227 transformer 2 CG10128-PD 0 CG2199 CT7250 CG2199 24655153 0 Flybase FBgn0035213 Drosophila melanogaster taxon 7227 CG2199-PB 0 tra2 protein interacts with CG2199 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-tra2 Gal4 DNA binding domain (DBD) fused to tra2 tra2 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CG2199 Gal4 DNA activation domain (AD) fused to CG2199 CG2199 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between tra2 and CG2199 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.455949707 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 35244 CG15436 CT35500 CG15436 19920688 0 Flybase FBgn0031610 Drosophila melanogaster taxon 7227 CG15436-PA 0 ttk ttk/FTZ-F2 E(yan)100D l(3)02667 ftzf2/ttk l(3)j7B8 l(3)j2A1 CT36470 CT36468 CT36466 CG11558 1418/06 1396/14 1372/08 1325/15 1281/04 1260/10 1209/10 1209/05 1184/16 1119/04 1049/07 0702/07 0438/31 0250/25 0037/17 ttkp69 schmal ftz-f2 FTZ-F2 CT5673 CG1856 ttk69 Ttk69 TTK88 5311 5125 3540 ovs oss osn Ttk tramtrack ttk 24651713 0 Flybase FBgn0003870 Drosophila melanogaster taxon 7227 tramtrack CG1856-PC 0 CG15436 protein interacts with ttk protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-CG15436 Gal4 DNA activation domain (AD) fused to CG15436 CG15436 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-ttk Gal4 DNA binding domain (DBD) fused to ttk ttk was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG15436 and ttk was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.303615185 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 37883 ttk ttk/FTZ-F2 E(yan)100D l(3)02667 ftzf2/ttk l(3)j7B8 l(3)j2A1 CT36470 CT36468 CT36466 CG11558 1418/06 1396/14 1372/08 1325/15 1281/04 1260/10 1209/10 1209/05 1184/16 1119/04 1049/07 0702/07 0438/31 0250/25 0037/17 ttkp69 schmal ftz-f2 FTZ-F2 CT5673 CG1856 ttk69 Ttk69 TTK88 5311 5125 3540 ovs oss osn Ttk tramtrack ttk 24651713 0 Flybase FBgn0003870 Drosophila melanogaster taxon 7227 tramtrack CG1856-PC 0 Arr1 Dmarrestin CT17964 DmArr1 CG5711 DS-Ag arr1 PRII ArrA 3H10 Arr DA Arrestin 1 Arr1 17136400 0 Flybase FBgn0000120 Drosophila melanogaster taxon 7227 Arrestin 1 CG5711-PA 0 ttk protein interacts with Arr1 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-ttk Gal4 DNA binding domain (DBD) fused to ttk ttk was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-Arr1 Gal4 DNA activation domain (AD) fused to Arr1 Arr1 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between ttk and Arr1 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.251440561 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 25235 tra2 CT28507 CG10128 tra-2 TRA-2 Tra2 transformer 2 tra2 19550175 0 Flybase FBgn0003742 Drosophila melanogaster taxon 7227 transformer 2 CG10128-PD 0 Tequila CT40927 CT15880 CT15447 CG18403 CG4948 CG4821 graal Tequila Drosophila melanogaster taxon 7227 CG4821-PB The cDNA isolated for this molecule contained only 3' untranslated sequence corresponding to GI 24661371 (Table S7, Supplementary Table). 0 tra2 protein interacts with Tequila protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). The cDNA isolated for Tequila contained only 3' untranslated sequence (Table S7, Supplementary Table). 0 0 34 Gal4-AD-tra2 Gal4 DNA activation domain (AD) fused to tra2 tra2 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-Tequila Gal4 DNA binding domain (DBD) fused to Tequila Tequila was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. The cDNA isolated for Tequila contained only 3' untranslated sequence (Table S7, Supplementary Table) 0 An interaction between tra2 and Tequila was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.061582474 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 29037 arm beta-catenin l(1)G0234 l(1)G0192 b-catenin EG:86E4.6 l(1)arm l(1)2Bv catenin CT36522 CT36518 CT12773 CG11579 Arm ARM armadillo arm 24639206 0 Flybase FBgn0000117 Drosophila melanogaster taxon 7227 armadillo CG11579-PC 0 CG3402 CT11431 CG3402 19923004 0 Flybase FBgn0035148 Drosophila melanogaster taxon 7227 CG3402-PA 0 arm protein interacts with CG3402 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-AD-arm Gal4 DNA activation domain (AD) fused to arm arm was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG3402 Gal4 DNA binding domain (DBD) fused to CG3402 CG3402 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between arm and CG3402 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.972141423 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 41112 CG3323 CT11173 CG3323 24639822 0 Flybase FBgn0029750 Drosophila melanogaster taxon 7227 CG3323-PA 0 cact n(2)k17003 CT18347 CG5848 dip6 cac cactus cact 24584588 0 Flybase FBgn0000250 Drosophila melanogaster taxon 7227 cactus CG5848-PA 0 CG3323 protein interacts with cact protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-CG3323 Gal4 DNA binding domain (DBD) fused to CG3323 CG3323 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-cact Gal4 DNA activation domain (AD) fused to cact cact was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG3323 and cact was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.106927184 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 37882 ttk ttk/FTZ-F2 E(yan)100D l(3)02667 ftzf2/ttk l(3)j7B8 l(3)j2A1 CT36470 CT36468 CT36466 CG11558 1418/06 1396/14 1372/08 1325/15 1281/04 1260/10 1209/10 1209/05 1184/16 1119/04 1049/07 0702/07 0438/31 0250/25 0037/17 ttkp69 schmal ftz-f2 FTZ-F2 CT5673 CG1856 ttk69 Ttk69 TTK88 5311 5125 3540 ovs oss osn Ttk tramtrack ttk 24651713 0 Flybase FBgn0003870 Drosophila melanogaster taxon 7227 tramtrack CG1856-PC 0 dei Delilah CT17256 CG5441 del delilah dei 24650311 0 Flybase FBgn0008649 Drosophila melanogaster taxon 7227 delilah CG5441-PA 0 ttk protein interacts with dei protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-ttk Gal4 DNA binding domain (DBD) fused to ttk ttk was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-dei Gal4 DNA activation domain (AD) fused to dei dei was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between ttk and dei was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.110146594 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 37884 ttk ttk/FTZ-F2 E(yan)100D l(3)02667 ftzf2/ttk l(3)j7B8 l(3)j2A1 CT36470 CT36468 CT36466 CG11558 1418/06 1396/14 1372/08 1325/15 1281/04 1260/10 1209/10 1209/05 1184/16 1119/04 1049/07 0702/07 0438/31 0250/25 0037/17 ttkp69 schmal ftz-f2 FTZ-F2 CT5673 CG1856 ttk69 Ttk69 TTK88 5311 5125 3540 ovs oss osn Ttk tramtrack ttk 24651713 0 Flybase FBgn0003870 Drosophila melanogaster taxon 7227 tramtrack CG1856-PC 0 lolal l(2)kO2512 l(2)k02512 CT18040 batman EP0647 CG5738 Batman EP647 25/12 lola like lolal 24654834 0 Flybase FBgn0022238 Drosophila melanogaster taxon 7227 lola like CG5738-PD 0 ttk protein interacts with lolal protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-AD-ttk Gal4 DNA activation domain (AD) fused to ttk ttk was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-lolal Gal4 DNA binding domain (DBD) fused to lolal lolal was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between ttk and lolal was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.710110083 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 30374 Hsp83 ms(3)08445 Su(Raf)3A l(3)j5C2 E(sina)2 E(sev)3A DMHSP82 CT2354 CG1242 hsp90 hsp84 hsp83 hsp82 Hsp90 Hsp82 HSP90 HSP83 HSP82 ORF1 stc 83 Heat shock protein 83 Hsp83 17647529 0 Flybase FBgn0001233 Drosophila melanogaster taxon 7227 Heat shock protein 83 CG1242-PA 0 CG14235 CT33854 CG14235 24643379 0 Flybase FBgn0031066 Drosophila melanogaster taxon 7227 CG14235-PC 0 Hsp83 protein interacts with CG14235 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-Hsp83 Gal4 DNA activation domain (AD) fused to Hsp83 Hsp83 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG14235 Gal4 DNA binding domain (DBD) fused to CG14235 CG14235 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Hsp83 and CG14235 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.139140645 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 37269 EG:171D11.5 EG:171D11.5 CT13856 CG17778 23397595 0 Flybase FBgn0023534 Drosophila melanogaster taxon 7227 CG17778-PA 0 BicD CT20532 CG6605 Bic[D] Bic-D BIC-D Bicaudal D BicD 24584833 0 Flybase FBgn0000183 Drosophila melanogaster taxon 7227 Bicaudal D CG6605-PA 0 EG:171D11.5 protein interacts with BicD protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-EG:171D11.5 Gal4 DNA binding domain (DBD) fused to EG:171D11.5 EG:171D11.5 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-BicD Gal4 DNA activation domain (AD) fused to BicD BicD was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between EG:171D11.5 and BicD was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.227295649 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 29059 CG11581 CT36532 CG11581 24641921 0 Flybase FBgn0030540 Drosophila melanogaster taxon 7227 CG11581-PA 0 ea CT15808 c-SP24 CG4920 SP24 easter ea 24647107 0 Flybase FBgn0000533 Drosophila melanogaster taxon 7227 easter CG4920-PA 0 CG11581 protein interacts with ea protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-CG11581 Gal4 DNA binding domain (DBD) fused to CG11581 CG11581 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-ea Gal4 DNA activation domain (AD) fused to ea ea was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG11581 and ea was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.21681689 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 41226 Coprox l(2)k11018 l(2)k10617 CT11567 CG3433 Coproporphyrinogen oxidase Coprox 17864388 0 Flybase FBgn0021944 Drosophila melanogaster taxon 7227 Coproporphyrinogen oxidase CG3433-PA 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 Coprox protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-DBD-Coprox Gal4 DNA binding domain (DBD) fused to Coprox Coprox was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-dl Gal4 DNA activation domain (AD) fused to dl dl was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Coprox and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.889144648 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 38153 CG18854 CT43399 CT32359 CG18854 24583138 0 Flybase FBgn0042174 Drosophila melanogaster taxon 7227 CG18854-PB 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 CG18854 protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-CG18854 Gal4 DNA activation domain (AD) fused to CG18854 CG18854 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG18854 and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.072537193 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 33844 CG14534 CT34264 CG14534 28574582 0 Flybase FBgn0031957 Drosophila melanogaster taxon 7227 CG14534-PA 0 ttk ttk/FTZ-F2 E(yan)100D l(3)02667 ftzf2/ttk l(3)j7B8 l(3)j2A1 CT36470 CT36468 CT36466 CG11558 1418/06 1396/14 1372/08 1325/15 1281/04 1260/10 1209/10 1209/05 1184/16 1119/04 1049/07 0702/07 0438/31 0250/25 0037/17 ttkp69 schmal ftz-f2 FTZ-F2 CT5673 CG1856 ttk69 Ttk69 TTK88 5311 5125 3540 ovs oss osn Ttk tramtrack ttk 24651713 0 Flybase FBgn0003870 Drosophila melanogaster taxon 7227 tramtrack CG1856-PC 0 CG14534 protein interacts with ttk protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-CG14534 Gal4 DNA activation domain (AD) fused to CG14534 CG14534 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-ttk Gal4 DNA binding domain (DBD) fused to ttk ttk was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG14534 and ttk was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.377199397 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 43576 cact n(2)k17003 CT18347 CG5848 dip6 cac cactus cact 24584588 0 Flybase FBgn0000250 Drosophila melanogaster taxon 7227 cactus CG5848-PA 0 CG7264 CT22423 CG7264 21357483 0 Flybase FBgn0036214 Drosophila melanogaster taxon 7227 CG7264-PA 0 cact protein interacts with CG7264 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-cact Gal4 DNA activation domain (AD) fused to cact cact was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG7264 Gal4 DNA binding domain (DBD) fused to CG7264 CG7264 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between cact and CG7264 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.256198759 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 42206 CG4328 CT14151 CG4328 24663246 0 Flybase FBgn0036274 Drosophila melanogaster taxon 7227 CG4328-PA 0 Fmr1 EP(3)3517 CT20883 CT17036 AT24755 cg6203 CG6203 dmfr1 dfmr1 dFXR1 dFMRP dFMR1 dfxr dFXR FMR1 FXR Fragile-X-related Fmr1 24645602 0 Flybase FBgn0028734 Drosophila melanogaster taxon 7227 CG6203-PB 0 CG4328 protein interacts with Fmr1 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-CG4328 Gal4 DNA activation domain (AD) fused to CG4328 CG4328 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-Fmr1 Gal4 DNA binding domain (DBD) fused to Fmr1 Fmr1 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG4328 and Fmr1 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.340582163 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 35256 CG15436 CT35500 CG15436 19920688 0 Flybase FBgn0031610 Drosophila melanogaster taxon 7227 CG15436-PA 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 CG15436 protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-CG15436 Gal4 DNA activation domain (AD) fused to CG15436 CG15436 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG15436 and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.044198442 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 30380 Hsp83 ms(3)08445 Su(Raf)3A l(3)j5C2 E(sina)2 E(sev)3A DMHSP82 CT2354 CG1242 hsp90 hsp84 hsp83 hsp82 Hsp90 Hsp82 HSP90 HSP83 HSP82 ORF1 stc 83 Heat shock protein 83 Hsp83 17647529 0 Flybase FBgn0001233 Drosophila melanogaster taxon 7227 Heat shock protein 83 CG1242-PA 0 Hop l(2)k00616 EP(2)0418 CT9251 CG2720 dSTI1 dhop Sti1 Hsp70/Hsp90 organizing protein homolog Hop 17137540 0 Flybase FBgn0024352 Drosophila melanogaster taxon 7227 Hsp70/Hsp90 organizing protein homolog CG2720-PA 0 Hsp83 protein interacts with Hop protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-Hsp83 Gal4 DNA activation domain (AD) fused to Hsp83 Hsp83 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-Hop Gal4 DNA binding domain (DBD) fused to Hop Hop was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Hsp83 and Hop was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.076021059 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 44088 ovo shavenbaby fs(1)K1237 Shavenbaby Fs(1)K1237 Fs(1)K1103 Fs(1)K155 fs(1)M38 ovo/svb fs(1)M1 CT36311 CT21113 CG6824 svb Svb Ovo OVO ovo ovo 24639804 0 Flybase FBgn0003028 Drosophila melanogaster taxon 7227 ovo CG6824-PA 0 Sd dRanGAP CT28175 RanGAP CG9999 Scl SD Segregation distorter Sd Drosophila melanogaster taxon 7227 Segregation distorter CG9999-PA The cDNA isolated for this molecule contained only 3' untranslated sequence corresponding to GI 17136452 (Table S7, Supplementary Table). 0 ovo protein interacts with Sd protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). The cDNA isolated for Sd contained only 3' untranslated sequence (Table S7, Supplementary Table). 0 0 34 Gal4-AD-ovo Gal4 DNA activation domain (AD) fused to ovo ovo was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-Sd Gal4 DNA binding domain (DBD) fused to Sd Sd was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. The cDNA isolated for Sd contained only 3' untranslated sequence (Table S7, Supplementary Table) 0 An interaction between ovo and Sd was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.537180755 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 41743 CG3967 CT39420 CT13090 CG3967 24661593 0 Flybase FBgn0035989 Drosophila melanogaster taxon 7227 CG3967-PB 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 CG3967 protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-CG3967 Gal4 DNA activation domain (AD) fused to CG3967 CG3967 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG3967 and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.269320677 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 43575 cact n(2)k17003 CT18347 CG5848 dip6 cac cactus cact 24584588 0 Flybase FBgn0000250 Drosophila melanogaster taxon 7227 cactus CG5848-PA 0 CG6416 CT20016 CT20013 CT19984 CG6416 28574418 0 Flybase FBgn0035917 Drosophila melanogaster taxon 7227 CG6416-PJ 0 cact protein interacts with CG6416 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-cact Gal4 DNA activation domain (AD) fused to cact cact was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG6416 Gal4 DNA binding domain (DBD) fused to CG6416 CG6416 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between cact and CG6416 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.044857537 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 34421 EG:63B12.12 EG:63B12.12 CT34631 CG14818 18543251 0 Flybase FBgn0026088 Drosophila melanogaster taxon 7227 CG14818-PA 0 Jra l(2R)IA109 l(2)IA109 dm-Jun CT7577 CG2275 dAP-1 d-jun d-Jun d-JRA c-Jun D-jun D-Jun dJun dJra dJUN dJRA cJun Djun DJun DJUN AP-1 jun Jun AP1 Jun-related antigen Jra 24652319 0 Flybase FBgn0001291 Drosophila melanogaster taxon 7227 Jun-related antigen CG2275-PB 0 EG:63B12.12 protein interacts with Jra protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-EG:63B12.12 Gal4 DNA activation domain (AD) fused to EG:63B12.12 EG:63B12.12 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-Jra Gal4 DNA binding domain (DBD) fused to Jra Jra was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between EG:63B12.12 and Jra was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.119543733 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 40058 CG31756 24582699 0 Flybase FBgn0051756 Drosophila melanogaster taxon 7227 CG31756-PA 0 Khc l(2)k13314 l(2R)W12 l(2)W12 kinesin CT23616 CG7765 KIF5A Dmkin DmKHC kin khc Kin KHC DKH DK Kinesin heavy chain Khc 17136240 0 Flybase FBgn0001308 Drosophila melanogaster taxon 7227 Kinesin heavy chain CG7765-PA 0 CG31756 protein interacts with Khc protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-CG31756 Gal4 DNA binding domain (DBD) fused to CG31756 CG31756 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-Khc Gal4 DNA activation domain (AD) fused to Khc Khc was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG31756 and Khc was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.201281328 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 38696 Jra l(2R)IA109 l(2)IA109 dm-Jun CT7577 CG2275 dAP-1 d-jun d-Jun d-JRA c-Jun D-jun D-Jun dJun dJra dJUN dJRA cJun Djun DJun DJUN AP-1 jun Jun AP1 Jun-related antigen Jra 24652319 0 Flybase FBgn0001291 Drosophila melanogaster taxon 7227 Jun-related antigen CG2275-PB 0 CG5694 CT17954 CG5694 24583270 0 Flybase FBgn0032197 Drosophila melanogaster taxon 7227 CG5694-PB 0 Jra protein interacts with CG5694 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-Jra Gal4 DNA binding domain (DBD) fused to Jra Jra was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CG5694 Gal4 DNA activation domain (AD) fused to CG5694 CG5694 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Jra and CG5694 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.27974175 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 35993 Cyp6t1 CT4488 CG1644 6t1 Cyp6t1 19920414 0 Flybase FBgn0031182 Drosophila melanogaster taxon 7227 CG1644-PA 0 Khc l(2)k13314 l(2R)W12 l(2)W12 kinesin CT23616 CG7765 KIF5A Dmkin DmKHC kin khc Kin KHC DKH DK Kinesin heavy chain Khc 17136240 0 Flybase FBgn0001308 Drosophila melanogaster taxon 7227 Kinesin heavy chain CG7765-PA 0 Cyp6t1 protein interacts with Khc protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-Cyp6t1 Gal4 DNA binding domain (DBD) fused to Cyp6t1 Cyp6t1 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-Khc Gal4 DNA activation domain (AD) fused to Khc Khc was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Cyp6t1 and Khc was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.351415258 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 43574 cact n(2)k17003 CT18347 CG5848 dip6 cac cactus cact 24584588 0 Flybase FBgn0000250 Drosophila melanogaster taxon 7227 cactus CG5848-PA 0 CG6357 CT19866 CG6357 20129967 0 Flybase FBgn0033875 Drosophila melanogaster taxon 7227 CG6357-PA 0 cact protein interacts with CG6357 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-AD-cact Gal4 DNA activation domain (AD) fused to cact cact was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG6357 Gal4 DNA binding domain (DBD) fused to CG6357 CG6357 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between cact and CG6357 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.561095084 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27620 so CG11121 CT8839 somda old med mda ami Drl So sine oculis so 17136492 0 Flybase FBgn0003460 Drosophila melanogaster taxon 7227 sine oculis CG11121-PA 0 CG9083 CT26070 CG9083 22024283 0 Flybase FBgn0035077 Drosophila melanogaster taxon 7227 CG9083-PA 0 so protein interacts with CG9083 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-so Gal4 DNA binding domain (DBD) fused to so so was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CG9083 Gal4 DNA activation domain (AD) fused to CG9083 CG9083 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between so and CG9083 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.116298656 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 43577 cact n(2)k17003 CT18347 CG5848 dip6 cac cactus cact 24584588 0 Flybase FBgn0000250 Drosophila melanogaster taxon 7227 cactus CG5848-PA 0 CG7352 CT22625 CG7352 24645053 0 Flybase FBgn0037581 Drosophila melanogaster taxon 7227 CG7352-PA 0 cact protein interacts with CG7352 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-cact Gal4 DNA activation domain (AD) fused to cact cact was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG7352 Gal4 DNA binding domain (DBD) fused to CG7352 CG7352 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between cact and CG7352 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.41581918 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27619 so CG11121 CT8839 somda old med mda ami Drl So sine oculis so 17136492 0 Flybase FBgn0003460 Drosophila melanogaster taxon 7227 sine oculis CG11121-PA 0 Chc l(1)13Fb CT25886 l(1)VI CG9012 D-Chc chc CLH CHC Clathrin heavy chain Chc 24642310 0 Flybase FBgn0000319 Drosophila melanogaster taxon 7227 Clathrin heavy chain CG9012-PB 0 so protein interacts with Chc protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-so Gal4 DNA binding domain (DBD) fused to so so was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-Chc Gal4 DNA activation domain (AD) fused to Chc Chc was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between so and Chc was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.077268595 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 38539 CG2199 CT7250 CG2199 24655153 0 Flybase FBgn0035213 Drosophila melanogaster taxon 7227 CG2199-PB 0 Jra l(2R)IA109 l(2)IA109 dm-Jun CT7577 CG2275 dAP-1 d-jun d-Jun d-JRA c-Jun D-jun D-Jun dJun dJra dJUN dJRA cJun Djun DJun DJUN AP-1 jun Jun AP1 Jun-related antigen Jra 24652319 0 Flybase FBgn0001291 Drosophila melanogaster taxon 7227 Jun-related antigen CG2275-PB 0 CG2199 protein interacts with Jra protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-CG2199 Gal4 DNA activation domain (AD) fused to CG2199 CG2199 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-Jra Gal4 DNA binding domain (DBD) fused to Jra Jra was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG2199 and Jra was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.049724978 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 29033 arm beta-catenin l(1)G0234 l(1)G0192 b-catenin EG:86E4.6 l(1)arm l(1)2Bv catenin CT36522 CT36518 CT12773 CG11579 Arm ARM armadillo arm 24639206 0 Flybase FBgn0000117 Drosophila melanogaster taxon 7227 armadillo CG11579-PC 0 CG16719 CT37201 CG16719 24661826 0 Flybase FBgn0036029 Drosophila melanogaster taxon 7227 CG16719-PA 0 arm protein interacts with CG16719 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-arm Gal4 DNA activation domain (AD) fused to arm arm was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG16719 Gal4 DNA binding domain (DBD) fused to CG16719 CG16719 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between arm and CG16719 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.163179705 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 30371 Hsp83 ms(3)08445 Su(Raf)3A l(3)j5C2 E(sina)2 E(sev)3A DMHSP82 CT2354 CG1242 hsp90 hsp84 hsp83 hsp82 Hsp90 Hsp82 HSP90 HSP83 HSP82 ORF1 stc 83 Heat shock protein 83 Hsp83 17647529 0 Flybase FBgn0001233 Drosophila melanogaster taxon 7227 Heat shock protein 83 CG1242-PA 0 CG13423 CT32780 CG13423 20130175 0 Flybase FBgn0034513 Drosophila melanogaster taxon 7227 CG13423-PA 0 Hsp83 protein interacts with CG13423 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-Hsp83 Gal4 DNA activation domain (AD) fused to Hsp83 Hsp83 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG13423 Gal4 DNA binding domain (DBD) fused to CG13423 CG13423 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Hsp83 and CG13423 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.046037442 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 43990 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 CtBP l(3)03464 l(3)03463 EP(3)3528 l(3)87De RE61183 RE01030 CT36671 CT23129 0256/16 l(3)G9 CG7583 256/16 dCtBP G9 C-terminal Binding Protein CtBP 24646452 0 Flybase FBgn0020496 Drosophila melanogaster taxon 7227 C-terminal Binding Protein CG7583-PD 0 dl protein interacts with CtBP protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CtBP Gal4 DNA activation domain (AD) fused to CtBP CtBP was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between dl and CtBP was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.057400266 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27618 so CG11121 CT8839 somda old med mda ami Drl So sine oculis so 17136492 0 Flybase FBgn0003460 Drosophila melanogaster taxon 7227 sine oculis CG11121-PA 0 CG8991 CT25850 CG8991 28573873 0 Flybase FBgn0033654 Drosophila melanogaster taxon 7227 CG8991-PA 0 so protein interacts with CG8991 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-DBD-so Gal4 DNA binding domain (DBD) fused to so so was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CG8991 Gal4 DNA activation domain (AD) fused to CG8991 CG8991 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between so and CG8991 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.729565635 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 30372 Hsp83 ms(3)08445 Su(Raf)3A l(3)j5C2 E(sina)2 E(sev)3A DMHSP82 CT2354 CG1242 hsp90 hsp84 hsp83 hsp82 Hsp90 Hsp82 HSP90 HSP83 HSP82 ORF1 stc 83 Heat shock protein 83 Hsp83 17647529 0 Flybase FBgn0001233 Drosophila melanogaster taxon 7227 Heat shock protein 83 CG1242-PA 0 spag l(2)k12101 CT32952 CG13570 spaghetti spag 17864228 0 Flybase FBgn0015544 Drosophila melanogaster taxon 7227 spaghetti CG13570-PA 0 Hsp83 protein interacts with spag protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-AD-Hsp83 Gal4 DNA activation domain (AD) fused to Hsp83 Hsp83 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-spag Gal4 DNA binding domain (DBD) fused to spag spag was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Hsp83 and spag was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.987477876 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 43738 Fmr1 EP(3)3517 CT20883 CT17036 AT24755 cg6203 CG6203 dmfr1 dfmr1 dFXR1 dFMRP dFMR1 dfxr dFXR FMR1 FXR Fragile-X-related Fmr1 24645602 0 Flybase FBgn0028734 Drosophila melanogaster taxon 7227 CG6203-PB 0 Stam CT20283 CG6521 Dstam STAM Signal transducing adaptor molecule Stam 17137700 0 Flybase FBgn0027363 Drosophila melanogaster taxon 7227 Signal transducing adaptor molecule CG6521-PA 0 Fmr1 protein interacts with Stam protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-Fmr1 Gal4 DNA binding domain (DBD) fused to Fmr1 Fmr1 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-Stam Gal4 DNA activation domain (AD) fused to Stam Stam was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Fmr1 and Stam was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.187985339 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27621 so CG11121 CT8839 somda old med mda ami Drl So sine oculis so 17136492 0 Flybase FBgn0003460 Drosophila melanogaster taxon 7227 sine oculis CG11121-PA 0 eya cli:clift CT27024 CG9554 EY2-1 CLIFT cli Eya eyes absent eya 24582246 0 Flybase FBgn0000320 Drosophila melanogaster taxon 7227 eyes absent CG9554-PA 0 so protein interacts with eya protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-DBD-so Gal4 DNA binding domain (DBD) fused to so so was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-eya Gal4 DNA activation domain (AD) fused to eya eya was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between so and eya was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.965220187 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 30377 Hsp83 ms(3)08445 Su(Raf)3A l(3)j5C2 E(sina)2 E(sev)3A DMHSP82 CT2354 CG1242 hsp90 hsp84 hsp83 hsp82 Hsp90 Hsp82 HSP90 HSP83 HSP82 ORF1 stc 83 Heat shock protein 83 Hsp83 17647529 0 Flybase FBgn0001233 Drosophila melanogaster taxon 7227 Heat shock protein 83 CG1242-PA 0 Vha100-1 vha100-1 CT4886 CG1709 Vha100-1 Drosophila melanogaster taxon 7227 CG1709-PH 0 Hsp83 protein interacts with Vha100-1 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-Hsp83 Gal4 DNA activation domain (AD) fused to Hsp83 Hsp83 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-Vha100-1 Gal4 DNA binding domain (DBD) fused to Vha100-1 Vha100-1 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Hsp83 and Vha100-1 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.266651197 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 30547 EG:BACR37P7.5 EG:BACR37P7.5 CT32706 CG12470 20128797 0 Flybase FBgn0040371 Drosophila melanogaster taxon 7227 CG12470-PA 0 Fmr1 EP(3)3517 CT20883 CT17036 AT24755 cg6203 CG6203 dmfr1 dfmr1 dFXR1 dFMRP dFMR1 dfxr dFXR FMR1 FXR Fragile-X-related Fmr1 24645602 0 Flybase FBgn0028734 Drosophila melanogaster taxon 7227 CG6203-PB 0 EG:BACR37P7.5 protein interacts with Fmr1 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-EG:BACR37P7.5 Gal4 DNA activation domain (AD) fused to EG:BACR37P7.5 EG:BACR37P7.5 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-Fmr1 Gal4 DNA binding domain (DBD) fused to Fmr1 Fmr1 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between EG:BACR37P7.5 and Fmr1 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.070870938 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 43991 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 CG8965 CT25764 CG8965 19920776 0 Flybase FBgn0031745 Drosophila melanogaster taxon 7227 CG8965-PA 0 dl protein interacts with CG8965 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CG8965 Gal4 DNA activation domain (AD) fused to CG8965 CG8965 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between dl and CG8965 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.237556811 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 41805 CG40127 30923682 0 Flybase FBgn0058127 Drosophila melanogaster taxon 7227 CG40127-PA.3 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 CG40127 protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-CG40127 Gal4 DNA activation domain (AD) fused to CG40127 CG40127 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG40127 and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.035905851 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 29039 arm beta-catenin l(1)G0234 l(1)G0192 b-catenin EG:86E4.6 l(1)arm l(1)2Bv catenin CT36522 CT36518 CT12773 CG11579 Arm ARM armadillo arm 24639206 0 Flybase FBgn0000117 Drosophila melanogaster taxon 7227 armadillo CG11579-PC 0 Apc2 CT19406 d-APC2 D-APC2 CG6193 dApc2 dAPC2 E-APC apc2 APC2 APC Adenomatous polyposis coli tumor suppressor homolog 2 Apc2 17137670 0 Flybase FBgn0026598 Drosophila melanogaster taxon 7227 Adenomatous polyposis coli tumor suppressor homolog 2 CG6193-PA 0 arm protein interacts with Apc2 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-arm Gal4 DNA activation domain (AD) fused to arm arm was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-Apc2 Gal4 DNA binding domain (DBD) fused to Apc2 Apc2 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between arm and Apc2 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.299687428 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 25233 tra2 CT28507 CG10128 tra-2 TRA-2 Tra2 transformer 2 tra2 19550175 0 Flybase FBgn0003742 Drosophila melanogaster taxon 7227 transformer 2 CG10128-PD 0 Rbp1 CT38058 CG17136 Rbp-1 rrm1 rbp1 RRM1 RBP1 RNA-binding protein 1 Rbp1 24645771 0 Flybase FBgn0010252 Drosophila melanogaster taxon 7227 RNA-binding protein 1 CG17136-PB 0 tra2 protein interacts with Rbp1 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-AD-tra2 Gal4 DNA activation domain (AD) fused to tra2 tra2 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-Rbp1 Gal4 DNA binding domain (DBD) fused to Rbp1 Rbp1 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between tra2 and Rbp1 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.883364642 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 44204 Ela CT21722 CG7021 Elastin-like Ela 24649822 0 Flybase FBgn0013949 Drosophila melanogaster taxon 7227 Elastin-like CG7021-PB 0 chic profilin l(2)27/7 Profilin CT36595 CT27020 CG9553 sand Chic chi D88 Chi chickadee chic 24582073 0 Flybase FBgn0000308 Drosophila melanogaster taxon 7227 chickadee CG9553-PC 0 Ela protein interacts with chic protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-Ela Gal4 DNA binding domain (DBD) fused to Ela Ela was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-chic Gal4 DNA activation domain (AD) fused to chic chic was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Ela and chic was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.164756633 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 25105 emc ms(3)61CD l(3)j4E11 l(3)05592 l(3)04322 0977/09 0587/01 0203/10 0094/26 Dm0688 CT1010 CG1007 gov Emc Ach extra macrochaetae emc 24654863 0 Flybase FBgn0000575 Drosophila melanogaster taxon 7227 extra macrochaetae CG1007-PA 0 sc Hairy-wing EG:198A6.1 scalpha l(1)1Ba DROACS2 CT12777 CG3827 sis-b sc/T4 ascT4 sisb sisB T4 Sc Hw scute sc 17136616 0 Flybase FBgn0004170 Drosophila melanogaster taxon 7227 scute CG3827-PA 0 emc protein interacts with sc protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-DBD-emc Gal4 DNA binding domain (DBD) fused to emc emc was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-sc Gal4 DNA activation domain (AD) fused to sc sc was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between emc and sc was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.701900755 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 30201 th E(rpr)3-1 Diap-1/Th l(3)j5C8 l(3)72Dc CT43237 CT18339 CG12284 1065/03 0736/01 0626/11 0604/04 0489/15 0306/05 diap-1 dIAP-1 Diap-1 DIAP-2 DIAP-1 D-iap1 D-IAP1 diap1 dIAP1 Diap1 DIAP1 dIAP Iap1 DIAP IAP thread th 24664975 0 Flybase FBgn0003691 Drosophila melanogaster taxon 7227 thread CG12284-PC 0 CG4554 CT14700 CG4554 24658164 0 Flybase FBgn0034734 Drosophila melanogaster taxon 7227 CG4554-PA 0 th protein interacts with CG4554 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-th Gal4 DNA activation domain (AD) fused to th th was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG4554 Gal4 DNA binding domain (DBD) fused to CG4554 CG4554 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between th and CG4554 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.461550161 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 25132 Egfr l(2)57DEFa l(2)57EFa l(2)09261 l(2)05351 Elp-B1RB1 l(2)57Ea DER/EGFR torpedo top/flb top/DER Torpedo Ellipse DmHD-33 DER/top CT28361 CG10079 dEGFR1 c-erbB Elp-B1 D-EGFR dEGFR HD-33 Elp-1 Egf-r EK2-6 EGF-R Degfr D-Egf C-erb egfr EgfR EGFr EGFR top flb Elp Egf Der DER El Epidermal growth factor receptor Egfr 17136536 0 Flybase FBgn0003731 Drosophila melanogaster taxon 7227 Epidermal growth factor receptor CG10079-PB 0 veli CT23375 D-veli CG7662 veli 24649911 0 Flybase FBgn0039269 Drosophila melanogaster taxon 7227 CG7662-PB 0 Egfr protein interacts with veli protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-AD-Egfr Gal4 DNA activation domain (AD) fused to Egfr Egfr was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-veli Gal4 DNA binding domain (DBD) fused to veli veli was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Egfr and veli was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.609085385 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 25232 tra2 CT28507 CG10128 tra-2 TRA-2 Tra2 transformer 2 tra2 19550175 0 Flybase FBgn0003742 Drosophila melanogaster taxon 7227 transformer 2 CG10128-PD 0 Doa l(3)01705 DOA/CLK2 CT36957 CT4592 CG1658 doa Msu DOA Darkener of apricot Doa 28571918 0 Flybase FBgn0000480 Drosophila melanogaster taxon 7227 Darkener of apricot CG1658-PD 0 tra2 protein interacts with Doa protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-DBD-tra2 Gal4 DNA binding domain (DBD) fused to tra2 tra2 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-Doa Gal4 DNA activation domain (AD) fused to Doa Doa was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between tra2 and Doa was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.973566445 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 37887 ttk ttk/FTZ-F2 E(yan)100D l(3)02667 ftzf2/ttk l(3)j7B8 l(3)j2A1 CT36470 CT36468 CT36466 CG11558 1418/06 1396/14 1372/08 1325/15 1281/04 1260/10 1209/10 1209/05 1184/16 1119/04 1049/07 0702/07 0438/31 0250/25 0037/17 ttkp69 schmal ftz-f2 FTZ-F2 CT5673 CG1856 ttk69 Ttk69 TTK88 5311 5125 3540 ovs oss osn Ttk tramtrack ttk 24651713 0 Flybase FBgn0003870 Drosophila melanogaster taxon 7227 tramtrack CG1856-PC 0 a CT32873 CT20921 CG13505 CG6741 bran Arc arc a 24657721 0 Flybase FBgn0000008 Drosophila melanogaster taxon 7227 arc CG6741-PA 0 ttk protein interacts with a protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-DBD-ttk Gal4 DNA binding domain (DBD) fused to ttk ttk was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-a Gal4 DNA activation domain (AD) fused to a a was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between ttk and a was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.6103381 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 25231 tra2 CT28507 CG10128 tra-2 TRA-2 Tra2 transformer 2 tra2 19550175 0 Flybase FBgn0003742 Drosophila melanogaster taxon 7227 transformer 2 CG10128-PD 0 B52 B52/dSRp55 l(3)s2249 CT41998 CT30371 CG10851 dSRp55 MabB52 E(Dfd) SRp55 RSp55 rrm8 SR55 Rbp8 RRM8 E726 B52 28571701 0 Flybase FBgn0004587 Drosophila melanogaster taxon 7227 CG10851-PG 0 tra2 protein interacts with B52 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-tra2 Gal4 DNA binding domain (DBD) fused to tra2 tra2 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-B52 Gal4 DNA activation domain (AD) fused to B52 B52 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between tra2 and B52 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.420294905 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 37888 ttk ttk/FTZ-F2 E(yan)100D l(3)02667 ftzf2/ttk l(3)j7B8 l(3)j2A1 CT36470 CT36468 CT36466 CG11558 1418/06 1396/14 1372/08 1325/15 1281/04 1260/10 1209/10 1209/05 1184/16 1119/04 1049/07 0702/07 0438/31 0250/25 0037/17 ttkp69 schmal ftz-f2 FTZ-F2 CT5673 CG1856 ttk69 Ttk69 TTK88 5311 5125 3540 ovs oss osn Ttk tramtrack ttk 24651713 0 Flybase FBgn0003870 Drosophila melanogaster taxon 7227 tramtrack CG1856-PC 0 AP-50 CT42529 CT21823 CG7057 Dmu2 AP50 mu2 AP-50 28571823 0 Flybase FBgn0024832 Drosophila melanogaster taxon 7227 CG7057-PB 0 ttk protein interacts with AP-50 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-AD-ttk Gal4 DNA activation domain (AD) fused to ttk ttk was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-AP-50 Gal4 DNA binding domain (DBD) fused to AP-50 AP-50 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between ttk and AP-50 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.958774935 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 25130 Egfr l(2)57DEFa l(2)57EFa l(2)09261 l(2)05351 Elp-B1RB1 l(2)57Ea DER/EGFR torpedo top/flb top/DER Torpedo Ellipse DmHD-33 DER/top CT28361 CG10079 dEGFR1 c-erbB Elp-B1 D-EGFR dEGFR HD-33 Elp-1 Egf-r EK2-6 EGF-R Degfr D-Egf C-erb egfr EgfR EGFr EGFR top flb Elp Egf Der DER El Epidermal growth factor receptor Egfr 17136536 0 Flybase FBgn0003731 Drosophila melanogaster taxon 7227 Epidermal growth factor receptor CG10079-PB 0 CG2944 24585817 0 Flybase FBgn0026238 Drosophila melanogaster taxon 7227 CG2944-PF 0 Egfr protein interacts with CG2944 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-Egfr Gal4 DNA activation domain (AD) fused to Egfr Egfr was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG2944 Gal4 DNA binding domain (DBD) fused to CG2944 CG2944 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Egfr and CG2944 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.292770091 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 30378 Hsp83 ms(3)08445 Su(Raf)3A l(3)j5C2 E(sina)2 E(sev)3A DMHSP82 CT2354 CG1242 hsp90 hsp84 hsp83 hsp82 Hsp90 Hsp82 HSP90 HSP83 HSP82 ORF1 stc 83 Heat shock protein 83 Hsp83 17647529 0 Flybase FBgn0001233 Drosophila melanogaster taxon 7227 Heat shock protein 83 CG1242-PA 0 cpb Ancpbeta CT32517 CG17158 J capping protein beta cpb 17136938 0 Flybase FBgn0011570 Drosophila melanogaster taxon 7227 capping protein beta CG17158-PA 0 Hsp83 protein interacts with cpb protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-Hsp83 Gal4 DNA activation domain (AD) fused to Hsp83 Hsp83 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-cpb Gal4 DNA binding domain (DBD) fused to cpb cpb was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Hsp83 and cpb was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.197274954 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 38433 ci l(4)102ABc l(4)17 l(4)13 CT6641 CG2125 ci[D] Ci[D] ci-D ciD CiD CID Ci Ce cubitus interruptus ci 24638496 0 Flybase FBgn0004859 Drosophila melanogaster taxon 7227 cubitus interruptus CG2125-PA 0 Su(fu) CT18933 su(fu) CG6054 Sufu SuFu Suppressor of fused Su(fu) 24646369 0 Flybase FBgn0005355 Drosophila melanogaster taxon 7227 Suppressor of fused CG6054-PA 0 ci protein interacts with Su(fu) protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-AD-ci Gal4 DNA activation domain (AD) fused to ci ci was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-Su(fu) Gal4 DNA binding domain (DBD) fused to Su(fu) Su(fu) was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between ci and Su(fu) was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.915077718 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 11 2003 11 12 Public Release 45297 cact n(2)k17003 CT18347 CG5848 dip6 cac cactus cact 24584588 0 Flybase FBgn0000250 Drosophila melanogaster taxon 7227 cactus CG5848-PA 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 cact protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 1 0 34 Gal4-DBD-cact Gal4 DNA binding domain (DBD) fused to cact cact was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-dl Gal4 DNA activation domain (AD) fused to dl dl was part of Drosophila embryo and adult cDNA libraries or was pooled from Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 An interaction between cact and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.960734821 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 1 34 Gal4-AD-cact Gal 4 DNA activation domain (AD) fused to cact cact was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal 4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 An interaction between cact and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.960734821 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 43739 Fmr1 EP(3)3517 CT20883 CT17036 AT24755 cg6203 CG6203 dmfr1 dfmr1 dFXR1 dFMRP dFMR1 dfxr dFXR FMR1 FXR Fragile-X-related Fmr1 24645602 0 Flybase FBgn0028734 Drosophila melanogaster taxon 7227 CG6203-PB 0 CG7671 CT23143 CG7671 24647938 0 Flybase FBgn0038609 Drosophila melanogaster taxon 7227 CG7671-PA 0 Fmr1 protein interacts with CG7671 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-DBD-Fmr1 Gal4 DNA binding domain (DBD) fused to Fmr1 Fmr1 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CG7671 Gal4 DNA activation domain (AD) fused to CG7671 CG7671 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Fmr1 and CG7671 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.700895922 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 29034 arm beta-catenin l(1)G0234 l(1)G0192 b-catenin EG:86E4.6 l(1)arm l(1)2Bv catenin CT36522 CT36518 CT12773 CG11579 Arm ARM armadillo arm 24639206 0 Flybase FBgn0000117 Drosophila melanogaster taxon 7227 armadillo CG11579-PC 0 Pros28.1A CT35901 CG17268 Pros28.1A 21356137 0 Flybase FBgn0017557 Drosophila melanogaster taxon 7227 CG17268-PA 0 arm protein interacts with Pros28.1A protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-arm Gal4 DNA activation domain (AD) fused to arm arm was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-Pros28.1A Gal4 DNA binding domain (DBD) fused to Pros28.1A Pros28.1A was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between arm and Pros28.1A was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.397974139 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 37886 ttk ttk/FTZ-F2 E(yan)100D l(3)02667 ftzf2/ttk l(3)j7B8 l(3)j2A1 CT36470 CT36468 CT36466 CG11558 1418/06 1396/14 1372/08 1325/15 1281/04 1260/10 1209/10 1209/05 1184/16 1119/04 1049/07 0702/07 0438/31 0250/25 0037/17 ttkp69 schmal ftz-f2 FTZ-F2 CT5673 CG1856 ttk69 Ttk69 TTK88 5311 5125 3540 ovs oss osn Ttk tramtrack ttk 24651713 0 Flybase FBgn0003870 Drosophila melanogaster taxon 7227 tramtrack CG1856-PC 0 CG6486 CT20197 CG6486 24661084 0 Flybase FBgn0035922 Drosophila melanogaster taxon 7227 CG6486-PA 0 ttk protein interacts with CG6486 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-DBD-ttk Gal4 DNA binding domain (DBD) fused to ttk ttk was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CG6486 Gal4 DNA activation domain (AD) fused to CG6486 CG6486 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between ttk and CG6486 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.549943709 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 30370 Hsp83 ms(3)08445 Su(Raf)3A l(3)j5C2 E(sina)2 E(sev)3A DMHSP82 CT2354 CG1242 hsp90 hsp84 hsp83 hsp82 Hsp90 Hsp82 HSP90 HSP83 HSP82 ORF1 stc 83 Heat shock protein 83 Hsp83 17647529 0 Flybase FBgn0001233 Drosophila melanogaster taxon 7227 Heat shock protein 83 CG1242-PA 0 CG12811 CT31939 CG12811 24645581 0 Flybase FBgn0037779 Drosophila melanogaster taxon 7227 CG12811-PA 0 Hsp83 protein interacts with CG12811 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-Hsp83 Gal4 DNA activation domain (AD) fused to Hsp83 Hsp83 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG12811 Gal4 DNA binding domain (DBD) fused to CG12811 CG12811 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Hsp83 and CG12811 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.326165741 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 35770 CG15764 CT36020 CG15764 24639988 0 Flybase FBgn0029817 Drosophila melanogaster taxon 7227 CG15764-PA 0 BicD CT20532 CG6605 Bic[D] Bic-D BIC-D Bicaudal D BicD 24584833 0 Flybase FBgn0000183 Drosophila melanogaster taxon 7227 Bicaudal D CG6605-PA 0 CG15764 protein interacts with BicD protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-CG15764 Gal4 DNA binding domain (DBD) fused to CG15764 CG15764 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-BicD Gal4 DNA activation domain (AD) fused to BicD BicD was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG15764 and BicD was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.14947564 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 26462 CG10576 CT29664 CG10576 24658590 0 Flybase FBgn0035630 Drosophila melanogaster taxon 7227 CG10576-PB 0 so CG11121 CT8839 somda old med mda ami Drl So sine oculis so 17136492 0 Flybase FBgn0003460 Drosophila melanogaster taxon 7227 sine oculis CG11121-PA 0 CG10576 protein interacts with so protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-AD-CG10576 Gal4 DNA activation domain (AD) fused to CG10576 CG10576 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-so Gal4 DNA binding domain (DBD) fused to so so was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG10576 and so was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.619562743 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 41828 CG40199 30923794 0 Flybase FBgn0028697 Drosophila melanogaster taxon 7227 CG17420-PA 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 CG40199 protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-CG40199 Gal4 DNA activation domain (AD) fused to CG40199 CG40199 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG40199 and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.028626644 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 37890 ttk ttk/FTZ-F2 E(yan)100D l(3)02667 ftzf2/ttk l(3)j7B8 l(3)j2A1 CT36470 CT36468 CT36466 CG11558 1418/06 1396/14 1372/08 1325/15 1281/04 1260/10 1209/10 1209/05 1184/16 1119/04 1049/07 0702/07 0438/31 0250/25 0037/17 ttkp69 schmal ftz-f2 FTZ-F2 CT5673 CG1856 ttk69 Ttk69 TTK88 5311 5125 3540 ovs oss osn Ttk tramtrack ttk 24651713 0 Flybase FBgn0003870 Drosophila melanogaster taxon 7227 tramtrack CG1856-PC 0 CG7386 CT22737 CG7386 24659237 0 Flybase FBgn0035691 Drosophila melanogaster taxon 7227 CG7386-PA 0 ttk protein interacts with CG7386 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-DBD-ttk Gal4 DNA binding domain (DBD) fused to ttk ttk was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CG7386 Gal4 DNA activation domain (AD) fused to CG7386 CG7386 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between ttk and CG7386 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.500316726 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 29035 arm beta-catenin l(1)G0234 l(1)G0192 b-catenin EG:86E4.6 l(1)arm l(1)2Bv catenin CT36522 CT36518 CT12773 CG11579 Arm ARM armadillo arm 24639206 0 Flybase FBgn0000117 Drosophila melanogaster taxon 7227 armadillo CG11579-PC 0 CG17319 CT38314 CG17319 Drosophila melanogaster taxon 7227 CG17319-PA The cDNA isolated for this molecule contained only 3' untranslated sequence corresponding to GI 24646478 (Table S7, Supplementary Table). 0 arm protein interacts with CG17319 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). The cDNA isolated for CG17319 contained only 3' untranslated sequence (Table S7, Supplementary Table). 0 0 34 Gal4-AD-arm Gal4 DNA activation domain (AD) fused to arm arm was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG17319 Gal4 DNA binding domain (DBD) fused to CG17319 CG17319 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. The cDNA isolated for CG17319 contained only 3' untranslated sequence (Table S7, Supplementary Table) 0 An interaction between arm and CG17319 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.051678245 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 42084 Stat92E l(3)06346 l(3)j6C8 Dstat92E stat92E marelle SD-stat DRODSRC CT13948 d-STAT D-stat D-Stat D-STAT CG4257 dSTAT Dstat DSTAT stat Stat STAT DSRC mrl Signal-transducer and activator of transcription protein at 92E Stat92E 28572156 0 Flybase FBgn0016917 Drosophila melanogaster taxon 7227 Signal-transducer and activator of transcription protein at 92E CG4257-PD 0 BG:DS06874.2 20129527 0 Flybase FBgn0028869 Drosophila melanogaster taxon 7227 CG4697-PA 0 Stat92E protein interacts with BG:DS06874.2 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-Stat92E Gal4 DNA activation domain (AD) fused to Stat92E Stat92E was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-BG:DS06874.2 Gal4 DNA binding domain (DBD) fused to BG:DS06874.2 BG:DS06874.2 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Stat92E and BG:DS06874.2 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.230074503 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 29040 arm beta-catenin l(1)G0234 l(1)G0192 b-catenin EG:86E4.6 l(1)arm l(1)2Bv catenin CT36522 CT36518 CT12773 CG11579 Arm ARM armadillo arm 24639206 0 Flybase FBgn0000117 Drosophila melanogaster taxon 7227 armadillo CG11579-PC 0 Ptp4E CT21187 DPTP4E CG6899 PTP4E Protein tyrosine phosphatase 4E Ptp4E 24639792 0 Flybase FBgn0004368 Drosophila melanogaster taxon 7227 Protein tyrosine phosphatase 4E CG6899-PB 0 arm protein interacts with Ptp4E protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-arm Gal4 DNA activation domain (AD) fused to arm arm was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-Ptp4E Gal4 DNA binding domain (DBD) fused to Ptp4E Ptp4E was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between arm and Ptp4E was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.21011938 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 30381 Hsp83 ms(3)08445 Su(Raf)3A l(3)j5C2 E(sina)2 E(sev)3A DMHSP82 CT2354 CG1242 hsp90 hsp84 hsp83 hsp82 Hsp90 Hsp82 HSP90 HSP83 HSP82 ORF1 stc 83 Heat shock protein 83 Hsp83 17647529 0 Flybase FBgn0001233 Drosophila melanogaster taxon 7227 Heat shock protein 83 CG1242-PA 0 Dpit47 CT10693 CG3189 DNA polymerase interacting tpr containing protein of 47kD Dpit47 17933624 0 Flybase FBgn0033083 Drosophila melanogaster taxon 7227 DNA polymerase interacting tpr containing protein of 47kD CG3189-PA 0 Hsp83 protein interacts with Dpit47 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-Hsp83 Gal4 DNA activation domain (AD) fused to Hsp83 Hsp83 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-Dpit47 Gal4 DNA binding domain (DBD) fused to Dpit47 Dpit47 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Hsp83 and Dpit47 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.266651197 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 30200 th E(rpr)3-1 Diap-1/Th l(3)j5C8 l(3)72Dc CT43237 CT18339 CG12284 1065/03 0736/01 0626/11 0604/04 0489/15 0306/05 diap-1 dIAP-1 Diap-1 DIAP-2 DIAP-1 D-iap1 D-IAP1 diap1 dIAP1 Diap1 DIAP1 dIAP Iap1 DIAP IAP thread th 24664975 0 Flybase FBgn0003691 Drosophila melanogaster taxon 7227 thread CG12284-PC 0 CG32772 CT35541 CG15469 CG6911 CG32772 24639782 0 Flybase FBgn0052772 Drosophila melanogaster taxon 7227 CG32772-PA 0 th protein interacts with CG32772 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-th Gal4 DNA activation domain (AD) fused to th th was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG32772 Gal4 DNA binding domain (DBD) fused to CG32772 CG32772 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between th and CG32772 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.159888129 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 44531 Khc l(2)k13314 l(2R)W12 l(2)W12 kinesin CT23616 CG7765 KIF5A Dmkin DmKHC kin khc Kin KHC DKH DK Kinesin heavy chain Khc 17136240 0 Flybase FBgn0001308 Drosophila melanogaster taxon 7227 Kinesin heavy chain CG7765-PA 0 sina CT28005 CG9949 sia seven in absentia sina 24665579 0 Flybase FBgn0003410 Drosophila melanogaster taxon 7227 seven in absentia CG9949-PB 0 Khc protein interacts with sina protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-Khc Gal4 DNA activation domain (AD) fused to Khc Khc was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-sina Gal4 DNA binding domain (DBD) fused to sina sina was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Khc and sina was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.072284719 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 30379 Hsp83 ms(3)08445 Su(Raf)3A l(3)j5C2 E(sina)2 E(sev)3A DMHSP82 CT2354 CG1242 hsp90 hsp84 hsp83 hsp82 Hsp90 Hsp82 HSP90 HSP83 HSP82 ORF1 stc 83 Heat shock protein 83 Hsp83 17647529 0 Flybase FBgn0001233 Drosophila melanogaster taxon 7227 Heat shock protein 83 CG1242-PA 0 CG17568 CT35085 CG17568 24585156 0 Flybase FBgn0032763 Drosophila melanogaster taxon 7227 CG17568-PA 0 Hsp83 protein interacts with CG17568 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-Hsp83 Gal4 DNA activation domain (AD) fused to Hsp83 Hsp83 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG17568 Gal4 DNA binding domain (DBD) fused to CG17568 CG17568 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Hsp83 and CG17568 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.03826946 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 44136 CG6905 CT42903 CT21370 CG6905 19922992 0 Flybase FBgn0035136 Drosophila melanogaster taxon 7227 CG6905-PA 0 Khc l(2)k13314 l(2R)W12 l(2)W12 kinesin CT23616 CG7765 KIF5A Dmkin DmKHC kin khc Kin KHC DKH DK Kinesin heavy chain Khc 17136240 0 Flybase FBgn0001308 Drosophila melanogaster taxon 7227 Kinesin heavy chain CG7765-PA 0 CG6905 protein interacts with Khc protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-CG6905 Gal4 DNA binding domain (DBD) fused to CG6905 CG6905 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-Khc Gal4 DNA activation domain (AD) fused to Khc Khc was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG6905 and Khc was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.086881738 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 43686 spz spaetzle mel(3)7 CT19282 CG6134 spatzle spz 24650508 0 Flybase FBgn0003495 Drosophila melanogaster taxon 7227 spatzle CG6134-PD 0 CG7940 CT23942 CG7940 21357129 0 Flybase FBgn0038576 Drosophila melanogaster taxon 7227 CG7940-PA 0 spz protein interacts with CG7940 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-AD-spz Gal4 DNA activation domain (AD) fused to spz spz was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG7940 Gal4 DNA binding domain (DBD) fused to CG7940 CG7940 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between spz and CG7940 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.783760589 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 30224 polo l(3)01673 POLO/PLK1 l(3)77Aa CT20542 CG12306 1324/08 0256/04 polo polo 24667346 0 Flybase FBgn0003124 Drosophila melanogaster taxon 7227 polo CG12306-PA 0 Jra l(2R)IA109 l(2)IA109 dm-Jun CT7577 CG2275 dAP-1 d-jun d-Jun d-JRA c-Jun D-jun D-Jun dJun dJra dJUN dJRA cJun Djun DJun DJUN AP-1 jun Jun AP1 Jun-related antigen Jra 24652319 0 Flybase FBgn0001291 Drosophila melanogaster taxon 7227 Jun-related antigen CG2275-PB 0 polo protein interacts with Jra protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-polo Gal4 DNA activation domain (AD) fused to polo polo was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-Jra Gal4 DNA binding domain (DBD) fused to Jra Jra was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between polo and Jra was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.468338664 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 30384 Hsp83 ms(3)08445 Su(Raf)3A l(3)j5C2 E(sina)2 E(sev)3A DMHSP82 CT2354 CG1242 hsp90 hsp84 hsp83 hsp82 Hsp90 Hsp82 HSP90 HSP83 HSP82 ORF1 stc 83 Heat shock protein 83 Hsp83 17647529 0 Flybase FBgn0001233 Drosophila melanogaster taxon 7227 Heat shock protein 83 CG1242-PA 0 CG32635 CT36002 CG12727 CG32635 24641790 0 Flybase FBgn0052635 Drosophila melanogaster taxon 7227 CG32635-PA 0 Hsp83 protein interacts with CG32635 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-Hsp83 Gal4 DNA activation domain (AD) fused to Hsp83 Hsp83 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG32635 Gal4 DNA binding domain (DBD) fused to CG32635 CG32635 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Hsp83 and CG32635 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.064385897 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 29038 arm beta-catenin l(1)G0234 l(1)G0192 b-catenin EG:86E4.6 l(1)arm l(1)2Bv catenin CT36522 CT36518 CT12773 CG11579 Arm ARM armadillo arm 24639206 0 Flybase FBgn0000117 Drosophila melanogaster taxon 7227 armadillo CG11579-PC 0 CG4771 CT15353 CG4771 21355719 0 Flybase FBgn0039018 Drosophila melanogaster taxon 7227 CG4771-PA 0 arm protein interacts with CG4771 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-arm Gal4 DNA activation domain (AD) fused to arm arm was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG4771 Gal4 DNA binding domain (DBD) fused to CG4771 CG4771 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between arm and CG4771 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.137884223 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 29201 Abd-B Uab-5[Sab] l(3)89Ed DROABDB CT40560 CT36635 CT28893 CG11648 CG10291 AbdB-II iab8.9 AbdB-I tuh-3 pH189 iab-8 iab-7 iab-6 iab-5 abd-B Fab-7 Abd-b twig iab9 iab8 iab7 iab6 iab5 abdB Fab7 AbdB ABDB iab Tab Sab Mcp MCP FAB Mc Abdominal B Abd-B 24647546 0 Flybase FBgn0000015 Drosophila melanogaster taxon 7227 Abdominal B CG11648-PD 0 Tdp1 CT25396 CT25394 CG8826 CG8825 gkt Tyrosyl-DNA phosophodiesterase 1 Tdp1 24581359 0 Flybase FBgn0031506 Drosophila melanogaster taxon 7227 Tyrosyl-DNA phosophodiesterase 1 CG8825-PA 0 Abd-B protein interacts with Tdp1 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-Abd-B Gal4 DNA binding domain (DBD) fused to Abd-B Abd-B was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-Tdp1 Gal4 DNA activation domain (AD) fused to Tdp1 Tdp1 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Abd-B and Tdp1 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.345621703 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 33376 slgA sluggish-A CT30657 CT30635 CT30603 CT3344 CG1417 PRODH EE85 tu3 slg sl sluggish A slgA 24643723 0 Flybase FBgn0003423 Drosophila melanogaster taxon 7227 sluggish A CG1417-PC 0 cact n(2)k17003 CT18347 CG5848 dip6 cac cactus cact 24584588 0 Flybase FBgn0000250 Drosophila melanogaster taxon 7227 cactus CG5848-PA 0 slgA protein interacts with cact protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-slgA Gal4 DNA binding domain (DBD) fused to slgA slgA was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-cact Gal4 DNA activation domain (AD) fused to cact cact was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between slgA and cact was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.214635332 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 37885 ttk ttk/FTZ-F2 E(yan)100D l(3)02667 ftzf2/ttk l(3)j7B8 l(3)j2A1 CT36470 CT36468 CT36466 CG11558 1418/06 1396/14 1372/08 1325/15 1281/04 1260/10 1209/10 1209/05 1184/16 1119/04 1049/07 0702/07 0438/31 0250/25 0037/17 ttkp69 schmal ftz-f2 FTZ-F2 CT5673 CG1856 ttk69 Ttk69 TTK88 5311 5125 3540 ovs oss osn Ttk tramtrack ttk 24651713 0 Flybase FBgn0003870 Drosophila melanogaster taxon 7227 tramtrack CG1856-PC 0 CG6459 CT20142 CG6459 20130085 0 Flybase FBgn0034259 Drosophila melanogaster taxon 7227 CG6459-PA 0 ttk protein interacts with CG6459 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-AD-ttk Gal4 DNA activation domain (AD) fused to ttk ttk was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG6459 Gal4 DNA binding domain (DBD) fused to CG6459 CG6459 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between ttk and CG6459 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.647847852 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 37891 ttk ttk/FTZ-F2 E(yan)100D l(3)02667 ftzf2/ttk l(3)j7B8 l(3)j2A1 CT36470 CT36468 CT36466 CG11558 1418/06 1396/14 1372/08 1325/15 1281/04 1260/10 1209/10 1209/05 1184/16 1119/04 1049/07 0702/07 0438/31 0250/25 0037/17 ttkp69 schmal ftz-f2 FTZ-F2 CT5673 CG1856 ttk69 Ttk69 TTK88 5311 5125 3540 ovs oss osn Ttk tramtrack ttk 24651713 0 Flybase FBgn0003870 Drosophila melanogaster taxon 7227 tramtrack CG1856-PC 0 BG:DS00180.10 BG:DS00180.10 CT25688 CG8942 19921312 0 Flybase FBgn0028545 Drosophila melanogaster taxon 7227 CG8942-PA 0 ttk protein interacts with BG:DS00180.10 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-ttk Gal4 DNA binding domain (DBD) fused to ttk ttk was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-BG:DS00180.10 Gal4 DNA activation domain (AD) fused to BG:DS00180.10 BG:DS00180.10 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between ttk and BG:DS00180.10 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.127160895 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 37164 Acp70A CT39049 CG17673 Spg70 Acp70 PapB ACP SP Accessory gland peptide 70A Acp70A 24663722 0 Flybase FBgn0003034 Drosophila melanogaster taxon 7227 Accessory gland peptide 70A CG17673-PA 0 Dl l(3)05151 l(3)j8C3 l(3)92Ab Overflow CT12133 1485/04 1440/11 1423/11 1304/03 1119/09 1053/14 0926/11 0495/20 CG3619 delta C1 D Delta Dl 24648166 0 Flybase FBgn0000463 Drosophila melanogaster taxon 7227 Delta CG3619-PB 0 Acp70A protein interacts with Dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-AD-Acp70A Gal4 DNA activation domain (AD) fused to Acp70A Acp70A was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-Dl Gal4 DNA binding domain (DBD) fused to Dl Dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Acp70A and Dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.791135706 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 26228 CG10470 CT29372 CG10470 24585112 0 Flybase FBgn0032746 Drosophila melanogaster taxon 7227 CG10470-PA 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 CG10470 protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-CG10470 Gal4 DNA activation domain (AD) fused to CG10470 CG10470 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG10470 and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.178935037 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 30382 Hsp83 ms(3)08445 Su(Raf)3A l(3)j5C2 E(sina)2 E(sev)3A DMHSP82 CT2354 CG1242 hsp90 hsp84 hsp83 hsp82 Hsp90 Hsp82 HSP90 HSP83 HSP82 ORF1 stc 83 Heat shock protein 83 Hsp83 17647529 0 Flybase FBgn0001233 Drosophila melanogaster taxon 7227 Heat shock protein 83 CG1242-PA 0 CG31999 fibrillin-like CG10323 CT7856 CG31999 28558748 0 Flybase FBgn0051999 Drosophila melanogaster taxon 7227 CG31999-PA 0 Hsp83 protein interacts with CG31999 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-Hsp83 Gal4 DNA activation domain (AD) fused to Hsp83 Hsp83 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG31999 Gal4 DNA binding domain (DBD) fused to CG31999 CG31999 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Hsp83 and CG31999 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.0421165 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 40704 CG32654 CT40576 CG18079 CG32654 24641567 0 Flybase FBgn0052654 Drosophila melanogaster taxon 7227 CG32654-PC 0 chic profilin l(2)27/7 Profilin CT36595 CT27020 CG9553 sand Chic chi D88 Chi chickadee chic 24582073 0 Flybase FBgn0000308 Drosophila melanogaster taxon 7227 chickadee CG9553-PC 0 CG32654 protein interacts with chic protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-CG32654 Gal4 DNA binding domain (DBD) fused to CG32654 CG32654 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-chic Gal4 DNA activation domain (AD) fused to chic chic was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG32654 and chic was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.106764082 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 39303 CG30327 CG30327 24657504 0 Flybase FBgn0050327 Drosophila melanogaster taxon 7227 CG30327-PA 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 CG30327 protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-CG30327 Gal4 DNA binding domain (DBD) fused to CG30327 CG30327 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-dl Gal4 DNA activation domain (AD) fused to dl dl was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG30327 and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.271645725 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27603 so CG11121 CT8839 somda old med mda ami Drl So sine oculis so 17136492 0 Flybase FBgn0003460 Drosophila melanogaster taxon 7227 sine oculis CG11121-PA 0 CG1218 CT2292 CG1218 21357321 0 Flybase FBgn0037377 Drosophila melanogaster taxon 7227 CG1218-PA 0 so protein interacts with CG1218 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-so Gal4 DNA binding domain (DBD) fused to so so was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-CG1218 Gal4 DNA activation domain (AD) fused to CG1218 CG1218 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between so and CG1218 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.303867006 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 29041 arm beta-catenin l(1)G0234 l(1)G0192 b-catenin EG:86E4.6 l(1)arm l(1)2Bv catenin CT36522 CT36518 CT12773 CG11579 Arm ARM armadillo arm 24639206 0 Flybase FBgn0000117 Drosophila melanogaster taxon 7227 armadillo CG11579-PC 0 CG7196 CT22211 CG7196 24582596 0 Flybase FBgn0031944 Drosophila melanogaster taxon 7227 CG7196-PB 0 arm protein interacts with CG7196 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-arm Gal4 DNA activation domain (AD) fused to arm arm was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG7196 Gal4 DNA binding domain (DBD) fused to CG7196 CG7196 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between arm and CG7196 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.075543035 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 29036 arm beta-catenin l(1)G0234 l(1)G0192 b-catenin EG:86E4.6 l(1)arm l(1)2Bv catenin CT36522 CT36518 CT12773 CG11579 Arm ARM armadillo arm 24639206 0 Flybase FBgn0000117 Drosophila melanogaster taxon 7227 armadillo CG11579-PC 0 pan l(4)102ABb LEF/TCF-1 TCF/LEF1 Tcf/LEF TCF/LEF LEF/TCF CT40034 CT40032 CG17964 l(4)13 Tcf-1 LEF-1 lef1 dTcf dTCF cTCF DTcf DTCF Tcf TCF Pan IA5 pangolin pan 28558732 0 Flybase FBgn0019664 Drosophila melanogaster taxon 7227 pangolin CG17964-PB 0 arm protein interacts with pan protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-AD-arm Gal4 DNA activation domain (AD) fused to arm arm was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-pan Gal4 DNA binding domain (DBD) fused to pan pan was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between arm and pan was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.849016564 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 30385 Hsp83 ms(3)08445 Su(Raf)3A l(3)j5C2 E(sina)2 E(sev)3A DMHSP82 CT2354 CG1242 hsp90 hsp84 hsp83 hsp82 Hsp90 Hsp82 HSP90 HSP83 HSP82 ORF1 stc 83 Heat shock protein 83 Hsp83 17647529 0 Flybase FBgn0001233 Drosophila melanogaster taxon 7227 Heat shock protein 83 CG1242-PA 0 CG3335 CT11193 cg3335 CG3335 24661759 0 Flybase FBgn0036018 Drosophila melanogaster taxon 7227 CG3335-PA 0 Hsp83 protein interacts with CG3335 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-Hsp83 Gal4 DNA activation domain (AD) fused to Hsp83 Hsp83 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG3335 Gal4 DNA binding domain (DBD) fused to CG3335 CG3335 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Hsp83 and CG3335 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.078979201 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 25131 Egfr l(2)57DEFa l(2)57EFa l(2)09261 l(2)05351 Elp-B1RB1 l(2)57Ea DER/EGFR torpedo top/flb top/DER Torpedo Ellipse DmHD-33 DER/top CT28361 CG10079 dEGFR1 c-erbB Elp-B1 D-EGFR dEGFR HD-33 Elp-1 Egf-r EK2-6 EGF-R Degfr D-Egf C-erb egfr EgfR EGFr EGFR top flb Elp Egf Der DER El Epidermal growth factor receptor Egfr 17136536 0 Flybase FBgn0003731 Drosophila melanogaster taxon 7227 Epidermal growth factor receptor CG10079-PB 0 Tequila CT40927 CT15880 CT15447 CG18403 CG4948 CG4821 graal Tequila 24661371 0 Flybase FBgn0023479 Drosophila melanogaster taxon 7227 CG4821-PB 0 Egfr protein interacts with Tequila protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-Egfr Gal4 DNA activation domain (AD) fused to Egfr Egfr was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-Tequila Gal4 DNA binding domain (DBD) fused to Tequila Tequila was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Egfr and Tequila was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.240325927 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 35298 RpL27A RpL27a2 RpL27Ab RpL27Aa CT35506 CG15442 rpL27a RpL27a Ribosomal protein L27A RpL27A 17136880 0 Flybase FBgn0010410 Drosophila melanogaster taxon 7227 Ribosomal protein L27A CG15442-PA 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 RpL27A protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-RpL27A Gal4 DNA activation domain (AD) fused to RpL27A RpL27A was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between RpL27A and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.04310305 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 25226 Men Mdh-NADP CT38328 CT28469 CG10120 MDH Me ME Malic enzyme Men 24646388 0 Flybase FBgn0002719 Drosophila melanogaster taxon 7227 Malic enzyme CG10120-PA 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 Men protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-Men Gal4 DNA activation domain (AD) fused to Men Men was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Men and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.138121378 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 25236 Sec61beta DSec61beta l(2)07214 CT28511 CG10130 Sec61beta 21356199 0 Flybase FBgn0010638 Drosophila melanogaster taxon 7227 CG10130-PA 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 Sec61beta protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-Sec61beta Gal4 DNA activation domain (AD) fused to Sec61beta Sec61beta was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Sec61beta and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.178935037 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 30908 CG12680 CT35539 CG12680 24639796 0 Flybase FBgn0029740 Drosophila melanogaster taxon 7227 CG12680-PA 0 ttk ttk/FTZ-F2 E(yan)100D l(3)02667 ftzf2/ttk l(3)j7B8 l(3)j2A1 CT36470 CT36468 CT36466 CG11558 1418/06 1396/14 1372/08 1325/15 1281/04 1260/10 1209/10 1209/05 1184/16 1119/04 1049/07 0702/07 0438/31 0250/25 0037/17 ttkp69 schmal ftz-f2 FTZ-F2 CT5673 CG1856 ttk69 Ttk69 TTK88 5311 5125 3540 ovs oss osn Ttk tramtrack ttk 24651713 0 Flybase FBgn0003870 Drosophila melanogaster taxon 7227 tramtrack CG1856-PC 0 CG12680 protein interacts with ttk protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-CG12680 Gal4 DNA activation domain (AD) fused to CG12680 CG12680 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-ttk Gal4 DNA binding domain (DBD) fused to ttk ttk was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG12680 and ttk was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.31146626 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 34647 CG14933 CT34761 CG14933 24583743 0 Flybase FBgn0040968 Drosophila melanogaster taxon 7227 CG14933-PA 0 csw l(1)GA114 l(1)G0170 E(sev)1A Csw/Shp2 l(1)csw l(1)2Dd l(1)2Db CT43319 CT37554 CT13063 CG3954 19-106 Csw CSW corkscrew csw 24639268 0 Flybase FBgn0000382 Drosophila melanogaster taxon 7227 corkscrew CG3954-PC 0 CG14933 protein interacts with csw protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-AD-CG14933 Gal4 DNA activation domain (AD) fused to CG14933 CG14933 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-csw Gal4 DNA binding domain (DBD) fused to csw csw was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG14933 and csw was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.579821358 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 38087 CG18777 CT42998 CG18777 24659170 0 Flybase FBgn0042118 Drosophila melanogaster taxon 7227 CG18777-PA 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 CG18777 protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-CG18777 Gal4 DNA activation domain (AD) fused to CG18777 CG18777 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG18777 and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.244299185 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 41320 CG3557 CT11964 CG3557 19920576 0 Flybase FBgn0031423 Drosophila melanogaster taxon 7227 CG3557-PA 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 CG3557 protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-CG3557 Gal4 DNA activation domain (AD) fused to CG3557 CG3557 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG3557 and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.087110091 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 34451 CG14829 CT34645 CG14829 24659863 0 Flybase FBgn0035751 Drosophila melanogaster taxon 7227 CG14829-PA 0 Khc l(2)k13314 l(2R)W12 l(2)W12 kinesin CT23616 CG7765 KIF5A Dmkin DmKHC kin khc Kin KHC DKH DK Kinesin heavy chain Khc 17136240 0 Flybase FBgn0001308 Drosophila melanogaster taxon 7227 Kinesin heavy chain CG7765-PA 0 CG14829 protein interacts with Khc protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-CG14829 Gal4 DNA binding domain (DBD) fused to CG14829 CG14829 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-Khc Gal4 DNA activation domain (AD) fused to Khc Khc was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG14829 and Khc was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.137801894 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 26167 CG10423 CT29278 CG10423 24649976 0 Flybase FBgn0039300 Drosophila melanogaster taxon 7227 CG10423-PA 0 so CG11121 CT8839 somda old med mda ami Drl So sine oculis so 17136492 0 Flybase FBgn0003460 Drosophila melanogaster taxon 7227 sine oculis CG11121-PA 0 CG10423 protein interacts with so protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-CG10423 Gal4 DNA activation domain (AD) fused to CG10423 CG10423 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-so Gal4 DNA binding domain (DBD) fused to so so was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG10423 and so was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.065199161 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 30387 Hsp83 ms(3)08445 Su(Raf)3A l(3)j5C2 E(sina)2 E(sev)3A DMHSP82 CT2354 CG1242 hsp90 hsp84 hsp83 hsp82 Hsp90 Hsp82 HSP90 HSP83 HSP82 ORF1 stc 83 Heat shock protein 83 Hsp83 17647529 0 Flybase FBgn0001233 Drosophila melanogaster taxon 7227 Heat shock protein 83 CG1242-PA 0 CG6830 CT21165 CG6830 Drosophila melanogaster taxon 7227 CG6830-PA The cDNA isolated for this molecule contained only 3' untranslated sequence corresponding to GI 21356635 (Table S7, Supplementary Table). 0 Hsp83 protein interacts with CG6830 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). The cDNA isolated for CG6830 contained only 3' untranslated sequence (Table S7, Supplementary Table). 0 0 34 Gal4-AD-Hsp83 Gal4 DNA activation domain (AD) fused to Hsp83 Hsp83 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG6830 Gal4 DNA binding domain (DBD) fused to CG6830 CG6830 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. The cDNA isolated for CG6830 contained only 3' untranslated sequence (Table S7, Supplementary Table) 0 An interaction between Hsp83 and CG6830 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.076021059 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 41986 l(2)35Ea l(2)br47 CT12971 CG4148 l35Ea lethal (2) 35Ea l(2)35Ea 17933736 0 Flybase FBgn0001990 Drosophila melanogaster taxon 7227 lethal (2) 35Ea CG4148-PA 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 l(2)35Ea protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-l(2)35Ea Gal4 DNA activation domain (AD) fused to l(2)35Ea l(2)35Ea was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between l(2)35Ea and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.093376692 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 43708 CG6156 anon-FF CT19330 CT19304 CG6156 24647139 0 Flybase FBgn0038328 Drosophila melanogaster taxon 7227 CG6156-PB 0 Khc l(2)k13314 l(2R)W12 l(2)W12 kinesin CT23616 CG7765 KIF5A Dmkin DmKHC kin khc Kin KHC DKH DK Kinesin heavy chain Khc 17136240 0 Flybase FBgn0001308 Drosophila melanogaster taxon 7227 Kinesin heavy chain CG7765-PA 0 CG6156 protein interacts with Khc protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-CG6156 Gal4 DNA binding domain (DBD) fused to CG6156 CG6156 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-Khc Gal4 DNA activation domain (AD) fused to Khc Khc was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG6156 and Khc was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.046758418 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 35486 CG15580 CT35699 CG15580 24644500 0 Flybase FBgn0037398 Drosophila melanogaster taxon 7227 CG15580-PB 0 cact n(2)k17003 CT18347 CG5848 dip6 cac cactus cact 24584588 0 Flybase FBgn0000250 Drosophila melanogaster taxon 7227 cactus CG5848-PA 0 CG15580 protein interacts with cact protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-DBD-CG15580 Gal4 DNA binding domain (DBD) fused to CG15580 CG15580 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-cact Gal4 DNA activation domain (AD) fused to cact cact was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG15580 and cact was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.933455445 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 40927 CG33070 CG33070 28571410 0 Flybase FBgn0053070 Drosophila melanogaster taxon 7227 CG33070-PK 0 Stat92E l(3)06346 l(3)j6C8 Dstat92E stat92E marelle SD-stat DRODSRC CT13948 d-STAT D-stat D-Stat D-STAT CG4257 dSTAT Dstat DSTAT stat Stat STAT DSRC mrl Signal-transducer and activator of transcription protein at 92E Stat92E 28572156 0 Flybase FBgn0016917 Drosophila melanogaster taxon 7227 Signal-transducer and activator of transcription protein at 92E CG4257-PD 0 CG33070 protein interacts with Stat92E protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-CG33070 Gal4 DNA binding domain (DBD) fused to CG33070 CG33070 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-Stat92E Gal4 DNA activation domain (AD) fused to Stat92E Stat92E was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG33070 and Stat92E was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.129653889 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 29875 CG12063 CT4480 CG12063 24651653 0 Flybase FBgn0039851 Drosophila melanogaster taxon 7227 CG12063-PA 0 Khc l(2)k13314 l(2R)W12 l(2)W12 kinesin CT23616 CG7765 KIF5A Dmkin DmKHC kin khc Kin KHC DKH DK Kinesin heavy chain Khc 17136240 0 Flybase FBgn0001308 Drosophila melanogaster taxon 7227 Kinesin heavy chain CG7765-PA 0 CG12063 protein interacts with Khc protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-CG12063 Gal4 DNA binding domain (DBD) fused to CG12063 CG12063 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-Khc Gal4 DNA activation domain (AD) fused to Khc Khc was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG12063 and Khc was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.436796437 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 33521 Mer Dmerlin CT33843 CG14228 merlin Emr2 EMR2 Merlin Mer 17647629 0 Flybase FBgn0013951 Drosophila melanogaster taxon 7227 Merlin CG14228-PA 0 cact n(2)k17003 CT18347 CG5848 dip6 cac cactus cact 24584588 0 Flybase FBgn0000250 Drosophila melanogaster taxon 7227 cactus CG5848-PA 0 Mer protein interacts with cact protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-Mer Gal4 DNA binding domain (DBD) fused to Mer Mer was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-cact Gal4 DNA activation domain (AD) fused to cact cact was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Mer and cact was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.099438235 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 39512 EG:84H4.4 EG:84H4.4 CT10316 CG3081 23397623 0 Flybase FBgn0025613 Drosophila melanogaster taxon 7227 CG3081-PA 0 spz spaetzle mel(3)7 CT19282 CG6134 spatzle spz 24650508 0 Flybase FBgn0003495 Drosophila melanogaster taxon 7227 spatzle CG6134-PD 0 EG:84H4.4 protein interacts with spz protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-EG:84H4.4 Gal4 DNA binding domain (DBD) fused to EG:84H4.4 EG:84H4.4 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-spz Gal4 DNA activation domain (AD) fused to spz spz was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between EG:84H4.4 and spz was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.199823894 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 28222 RpS12 l(3)s2783 CT31457 CG17672 CG11271 RpS12E RS12 Ribosomal protein S12 RpS12 24663657 0 Flybase FBgn0014027 Drosophila melanogaster taxon 7227 Ribosomal protein S12 CG11271-PE 0 Abd-B Uab-5[Sab] l(3)89Ed DROABDB CT40560 CT36635 CT28893 CG11648 CG10291 AbdB-II iab8.9 AbdB-I tuh-3 pH189 iab-8 iab-7 iab-6 iab-5 abd-B Fab-7 Abd-b twig iab9 iab8 iab7 iab6 iab5 abdB Fab7 AbdB ABDB iab Tab Sab Mcp MCP FAB Mc Abdominal B Abd-B 24647546 0 Flybase FBgn0000015 Drosophila melanogaster taxon 7227 Abdominal B CG11648-PD 0 RpS12 protein interacts with Abd-B protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-RpS12 Gal4 DNA activation domain (AD) fused to RpS12 RpS12 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-Abd-B Gal4 DNA binding domain (DBD) fused to Abd-B Abd-B was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between RpS12 and Abd-B was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.074311952 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 32883 Arc32 CT33389 CG13867 dARC32 Arc32 24415388 0 Flybase FBgn0034503 Drosophila melanogaster taxon 7227 CG13867-PA 0 dl CT42418 CT20694 CT20676 CG6667 dL dorsal dl 24584826 0 Flybase FBgn0000462 Drosophila melanogaster taxon 7227 dorsal CG6667-PC 0 Arc32 protein interacts with dl protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-Arc32 Gal4 DNA activation domain (AD) fused to Arc32 Arc32 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-dl Gal4 DNA binding domain (DBD) fused to dl dl was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Arc32 and dl was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.086551364 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 44628 CG7945 CT37755 CT23958 CG7945 24664652 0 Flybase FBgn0036505 Drosophila melanogaster taxon 7227 CG7945-PA 0 chic profilin l(2)27/7 Profilin CT36595 CT27020 CG9553 sand Chic chi D88 Chi chickadee chic 24582073 0 Flybase FBgn0000308 Drosophila melanogaster taxon 7227 chickadee CG9553-PC 0 CG7945 protein interacts with chic protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-CG7945 Gal4 DNA binding domain (DBD) fused to CG7945 CG7945 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-chic Gal4 DNA activation domain (AD) fused to chic chic was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between CG7945 and chic was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.187615113 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 41474 dock l(2)04723 CT42218 CT12313 Dm0447 CG3727 Dock doc dck dreadlocks dock 24580718 0 Flybase FBgn0010583 Drosophila melanogaster taxon 7227 dreadlocks CG3727-PC 0 Fmr1 EP(3)3517 CT20883 CT17036 AT24755 cg6203 CG6203 dmfr1 dfmr1 dFXR1 dFMRP dFMR1 dfxr dFXR FMR1 FXR Fragile-X-related Fmr1 24645602 0 Flybase FBgn0028734 Drosophila melanogaster taxon 7227 CG6203-PB 0 dock protein interacts with Fmr1 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a high confidence value (greater than 0.5). 0 0 34 Gal4-AD-dock Gal4 DNA activation domain (AD) fused to dock dock was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-Fmr1 Gal4 DNA binding domain (DBD) fused to Fmr1 Fmr1 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between dock and Fmr1 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a high confidence value of 0.681869448 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 27487 GM130 CT30999 CT30875 CG11061 dGM130 GM130 24657684 0 Flybase FBgn0034697 Drosophila melanogaster taxon 7227 CG11061-PA 0 cact n(2)k17003 CT18347 CG5848 dip6 cac cactus cact 24584588 0 Flybase FBgn0000250 Drosophila melanogaster taxon 7227 cactus CG5848-PA 0 GM130 protein interacts with cact protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-DBD-GM130 Gal4 DNA binding domain (DBD) fused to GM130 GM130 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-AD-cact Gal4 DNA activation domain (AD) fused to cact cact was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between GM130 and cact was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.318506806 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 30386 Hsp83 ms(3)08445 Su(Raf)3A l(3)j5C2 E(sina)2 E(sev)3A DMHSP82 CT2354 CG1242 hsp90 hsp84 hsp83 hsp82 Hsp90 Hsp82 HSP90 HSP83 HSP82 ORF1 stc 83 Heat shock protein 83 Hsp83 17647529 0 Flybase FBgn0001233 Drosophila melanogaster taxon 7227 Heat shock protein 83 CG1242-PA 0 Gli n(2)k09033 gliotactin l(2)br45 l(2)35Dg CT12983 CG3903 gli Gliotactin Gli Drosophila melanogaster taxon 7227 Gliotactin CG3903-PB The cDNA isolated for this molecule contained only 3' untranslated sequence corresponding to GI 24584490 (Table S7, Supplementary Table). 0 Hsp83 protein interacts with Gli protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). The cDNA isolated for Gli contained only 3' untranslated sequence (Table S7, Supplementary Table). 0 0 34 Gal4-AD-Hsp83 Gal4 DNA activation domain (AD) fused to Hsp83 Hsp83 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-Gli Gal4 DNA binding domain (DBD) fused to Gli Gli was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. The cDNA isolated for Gli contained only 3' untranslated sequence (Table S7, Supplementary Table) 0 An interaction between Hsp83 and Gli was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.050567649 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 43956 BicD CT20532 CG6605 Bic[D] Bic-D BIC-D Bicaudal D BicD 24584833 0 Flybase FBgn0000183 Drosophila melanogaster taxon 7227 Bicaudal D CG6605-PA 0 sip1 CT22315 CG7238 septin interacting protein 1 sip1 24582006 0 Flybase FBgn0024191 Drosophila melanogaster taxon 7227 septin interacting protein 1 CG7238-PA 0 BicD protein interacts with sip1 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-BicD Gal4 DNA activation domain (AD) fused to BicD BicD was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-sip1 Gal4 DNA binding domain (DBD) fused to sip1 sip1 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between BicD and sip1 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.314634108 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 42244 Pka-C1 l(2)s4402 l(2)01272 CT14278 pka-C1 PKA-C1 CG4379 Pka-C PKAcA dPKA PKAc Dcpk CdkA 6353 pka pkA pKA dco dcO Pka PKA Dco Dc0 DCO DC0 C cAMP-dependent protein kinase 1 Pka-C1 24583100 0 Flybase FBgn0000273 Drosophila melanogaster taxon 7227 cAMP-dependent protein kinase 1 CG4379-PB 0 cnn oblivious CT41755 CT15503 CG18370 CG4832 obv mfs Cnn CNN centrosomin cnn Drosophila melanogaster taxon 7227 centrosomin CG4832-PC The cDNA isolated for this molecule contained only 3' untranslated sequence corresponding to GI 24653385 (Table S7, Supplementary Table). 0 Pka-C1 protein interacts with cnn protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). The cDNA isolated for cnn contained only 3' untranslated sequence (Table S7, Supplementary Table). 0 0 34 Gal4-AD-Pka-C1 Gal4 DNA activation domain (AD) fused to Pka-C1 Pka-C1 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-cnn Gal4 DNA binding domain (DBD) fused to cnn cnn was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. The cDNA isolated for cnn contained only 3' untranslated sequence (Table S7, Supplementary Table) 0 An interaction between Pka-C1 and cnn was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.090408104 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa 2003 11 12 2003 11 12 Public Release 30383 Hsp83 ms(3)08445 Su(Raf)3A l(3)j5C2 E(sina)2 E(sev)3A DMHSP82 CT2354 CG1242 hsp90 hsp84 hsp83 hsp82 Hsp90 Hsp82 HSP90 HSP83 HSP82 ORF1 stc 83 Heat shock protein 83 Hsp83 17647529 0 Flybase FBgn0001233 Drosophila melanogaster taxon 7227 Heat shock protein 83 CG1242-PA 0 CG3225 CT10799 cg3225 CG3225 19920696 0 Flybase FBgn0031631 Drosophila melanogaster taxon 7227 CG3225-PA 0 Hsp83 protein interacts with CG3225 protein in a high-throughput yeast 2-hybrid screen. This interaction was associated with a low confidence value (less than 0.5). 0 0 34 Gal4-AD-Hsp83 Gal4 DNA activation domain (AD) fused to Hsp83 Hsp83 was part of Drosophila embryo and adult cDNA libraries or was part of a pool of ORFs amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries. 0 Gal4-DBD-CG3225 Gal4 DNA binding domain (DBD) fused to CG3225 CG3225 was amplified by PCR from pooled Drosophila embryo, larval1, larval2, larval3, pupal and adult cDNA libraries 0 An interaction between Hsp83 and CG3225 was demonstrated by high-throughput yeast two-hybrid screen. Yeast cells transformed with bait fused to the Gal-4 DNA binding domain (DBD) were mated with yeast cells containing Drosophila cDNA libraries or a collection of PCR amplified ORFs fused to the Gal4 activation domain (AD). Interactions were detected by growth on media lacking uracil and beta-galactosidase activity. Interacting proteins were identified by DNA purification and sequencing of positive clones. This interaction was assigned a low confidence value of 0.034688427 Supplementary table S7, Supplementary Methods and Materials. A protein interaction map of Drosophila melanogaster. 14605208 A protein interaction map of Drosophila melanogaster. 14605208 Muskat Brenda Brenda Muskat BM Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Wolting Cheryl Cheryl Wolting CW Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 D'Abreo Cheryl Cheryl D'Abreo CD Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 Bobechko Brian Brian Bobechko BB Samuel Lunenfeld Research Institute, Blueprint Initiative, Mt. Sinai Hospital, 600 University Avenue, Toronto ON, Canada, M5G 1X5; Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto ON, Canada M5S 1A8 BIND Metazoa